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Quantitative Imagery Analysis of Spot Patterns for Haplogroup Classification of Triatoma Dimidiata (Latreille, 1811) (Hemiptera: Reduviidae) an Important Vector of Chagas Disease
Daryl David Cruz Flores
Centro de Investigación en Biodiversidad y Conservación
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7714-2459
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Spots and coloring patterns evaluated quantitatively can be used to discrimination and identify possible cryptic species. Triatoma dimidiata (Reduviidae: Triatominae) is one of the main complexes of Chagas disease vector species. Phylogenetic studies have defined three haplogroups for Mexico and part of Central America. In this work, we evaluate the possibility of correctly discriminating among these T. dimidiata haplogroups using the pattern of dorsal spots.
Methods: Digital images of the dorsal region of individuals from the three haplogroups were used. We used image processing to extract primary and secondary variables characterizing the dorsal spot pattern. The statistical analysis of the variables included descriptive statistics, non-parametric Kruskal-Wallis tests, Discriminant Function Analysis (DFA), and a neural classification network.
Results: A distinctive spot pattern was found for each haplogroup. The most differentiated pattern was presented by haplogroup 2, mainly apparent in the notably larger central spots. Haplogroups 1 and 3 were more similar to each other, but there were consistent differences in the shape and orientation of the spots. Significant differences were found among haplogroups in almost all the variables analyzed, these differences being greater in relative spot area, mean relative area of central spots, central spots Feret diameters, lateral spots Feret diameters and aspect ratio. Both the DFA and the neural network had correct discrimination values above 90%.
Conclusions: We conclude that the spot pattern can be reliably used to discriminate among haplogroups, and possibly among triatomine species.
Keywords
cryptic species, coloring pattern, species complex, taxonomy, triatomine
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This preprint is under consideration at Parasites & Vectors. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Quantitative Imagery Analysis of Spot Patterns for Haplogroup Classification of Triatoma Dimidiata (Latreille, 1811) (Hemiptera: Reduviidae) an Important Vector of Chagas Disease
Daryl David Cruz Flores
Centro de Investigación en Biodiversidad y Conservación
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7714-2459
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version (no preprint)
Posted 01 Jan, 2021
Editor invited
On 01 Jan, 2021
Editor assigned
On 01 Jan, 2021
Submission checks complete
On 01 Jan, 2021
This version was not preprinted
Version (no preprint)
Posted 28 Dec, 2020
Review #1 received
Received 28 Dec, 2020
Review #2 received
Received 27 Dec, 2020
Reviewer #2 agreed
On 14 Dec, 2020
Reviewer #1 agreed
On 12 Dec, 2020
Reviewers invited
Invitations sent on 11 Dec, 2020
Editor invited
On 01 Dec, 2020
Editor assigned
On 01 Dec, 2020
Submission checks complete
On 01 Dec, 2020
This version was not preprinted
Version 1
Posted 18 Sep, 2020
No community comments so far
Editorial decision: Major Revision
On 12 Nov, 2020
Review #1 received
Received 11 Nov, 2020
Review #2 received
Received 16 Oct, 2020
Reviewer #2 agreed
On 01 Oct, 2020
Reviewer #1 agreed
On 26 Sep, 2020
Reviewers invited
Invitations sent on 21 Sep, 2020
First submitted
On 16 Sep, 2020
Editor invited
On 16 Sep, 2020
Editor assigned
On 16 Sep, 2020
Submission checks complete
On 15 Sep, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Spots and coloring patterns evaluated quantitatively can be used to discrimination and identify possible cryptic species. Triatoma dimidiata (Reduviidae: Triatominae) is one of the main complexes of Chagas disease vector species. Phylogenetic studies have defined three haplogroups for Mexico and part of Central America. In this work, we evaluate the possibility of correctly discriminating among these T. dimidiata haplogroups using the pattern of dorsal spots.
Methods: Digital images of the dorsal region of individuals from the three haplogroups were used. We used image processing to extract primary and secondary variables characterizing the dorsal spot pattern. The statistical analysis of the variables included descriptive statistics, non-parametric Kruskal-Wallis tests, Discriminant Function Analysis (DFA), and a neural classification network.
Results: A distinctive spot pattern was found for each haplogroup. The most differentiated pattern was presented by haplogroup 2, mainly apparent in the notably larger central spots. Haplogroups 1 and 3 were more similar to each other, but there were consistent differences in the shape and orientation of the spots. Significant differences were found among haplogroups in almost all the variables analyzed, these differences being greater in relative spot area, mean relative area of central spots, central spots Feret diameters, lateral spots Feret diameters and aspect ratio. Both the DFA and the neural network had correct discrimination values above 90%.
Conclusions: We conclude that the spot pattern can be reliably used to discriminate among haplogroups, and possibly among triatomine species.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10