Quantitative Imagery Analysis of Spot Patterns for Haplogroup Classification of Triatoma Dimidiata (Latreille, 1811) (Hemiptera: Reduviidae) an Important Vector of Chagas Disease
Background: Spots and coloring patterns evaluated quantitatively can be used to discrimination and identify possible cryptic species. Triatoma dimidiata (Reduviidae: Triatominae) is one of the main complexes of Chagas disease vector species. Phylogenetic studies have defined three haplogroups for Mexico and part of Central America. In this work, we evaluate the possibility of correctly discriminating among these T. dimidiata haplogroups using the pattern of dorsal spots.
Methods: Digital images of the dorsal region of individuals from the three haplogroups were used. We used image processing to extract primary and secondary variables characterizing the dorsal spot pattern. The statistical analysis of the variables included descriptive statistics, non-parametric Kruskal-Wallis tests, Discriminant Function Analysis (DFA), and a neural classification network.
Results: A distinctive spot pattern was found for each haplogroup. The most differentiated pattern was presented by haplogroup 2, mainly apparent in the notably larger central spots. Haplogroups 1 and 3 were more similar to each other, but there were consistent differences in the shape and orientation of the spots. Significant differences were found among haplogroups in almost all the variables analyzed, these differences being greater in relative spot area, mean relative area of central spots, central spots Feret diameters, lateral spots Feret diameters and aspect ratio. Both the DFA and the neural network had correct discrimination values above 90%.
Conclusions: We conclude that the spot pattern can be reliably used to discriminate among haplogroups, and possibly among triatomine species.
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Quantitative Imagery Analysis of Spot Patterns for Haplogroup Classification of Triatoma Dimidiata (Latreille, 1811) (Hemiptera: Reduviidae) an Important Vector of Chagas Disease
Posted 01 Jan, 2021
On 01 Jan, 2021
On 01 Jan, 2021
On 01 Jan, 2021
Posted 28 Dec, 2020
Received 28 Dec, 2020
Received 27 Dec, 2020
On 14 Dec, 2020
On 12 Dec, 2020
Invitations sent on 11 Dec, 2020
On 01 Dec, 2020
On 01 Dec, 2020
On 01 Dec, 2020
Posted 18 Sep, 2020
On 12 Nov, 2020
Received 11 Nov, 2020
Received 16 Oct, 2020
On 01 Oct, 2020
On 26 Sep, 2020
Invitations sent on 21 Sep, 2020
On 16 Sep, 2020
On 16 Sep, 2020
On 16 Sep, 2020
On 15 Sep, 2020
Background: Spots and coloring patterns evaluated quantitatively can be used to discrimination and identify possible cryptic species. Triatoma dimidiata (Reduviidae: Triatominae) is one of the main complexes of Chagas disease vector species. Phylogenetic studies have defined three haplogroups for Mexico and part of Central America. In this work, we evaluate the possibility of correctly discriminating among these T. dimidiata haplogroups using the pattern of dorsal spots.
Methods: Digital images of the dorsal region of individuals from the three haplogroups were used. We used image processing to extract primary and secondary variables characterizing the dorsal spot pattern. The statistical analysis of the variables included descriptive statistics, non-parametric Kruskal-Wallis tests, Discriminant Function Analysis (DFA), and a neural classification network.
Results: A distinctive spot pattern was found for each haplogroup. The most differentiated pattern was presented by haplogroup 2, mainly apparent in the notably larger central spots. Haplogroups 1 and 3 were more similar to each other, but there were consistent differences in the shape and orientation of the spots. Significant differences were found among haplogroups in almost all the variables analyzed, these differences being greater in relative spot area, mean relative area of central spots, central spots Feret diameters, lateral spots Feret diameters and aspect ratio. Both the DFA and the neural network had correct discrimination values above 90%.
Conclusions: We conclude that the spot pattern can be reliably used to discriminate among haplogroups, and possibly among triatomine species.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10