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Research
Sarika Tiwari
All India Institute of Medical Sciences Jodhpur India
Corresponding Author
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Background
Echovirus 30 (E30) causes acute aseptic meningitis. Enteroviruses (EVs) are responsible for 30,000 to 50,000 hospitalizations for aseptic meningitis per year in the United States. E30 is one of the most frequently isolated EVs, causing extensive outbreaks in temperate climates in several countries.
Methods
E30 used in this study was isolated from environmental specimens. The virus was confirmed by RT-PCR with specific primers (1). Virus stock was prepared by infecting RD cells in 25 cm2 flasks (Corning Inc. USA). Virus infected and mock infected cells were incubated at 36.5 ºC with 2% MEM. After 48 h of infection, the culture was aliquoted and kept at-80 ºC for further use.
Results
This present work analyzed the E30 genetic diversity in a fragment of 217 nucleotides of the VP1 region, of environmental strains. The environmental samples of echovirus 30 show mutation in the nonfunctional polyprotein protein (Fig. 1). The result suggested that the information may be obtained by analyzing only a part of the VP1 region.
Conclusion
The present study showed that the E30 environmental sample is more divergent to prototype Bastianni strain.
Keywords
Echovirus 30, Aseptic meningitis, Phylogenetic tree, Genetic diversity, VP1 region
Figure 1
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Sarika Tiwari
All India Institute of Medical Sciences Jodhpur India
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 18 Sep, 2020
Community comments: 1
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Virology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Echovirus 30 (E30) causes acute aseptic meningitis. Enteroviruses (EVs) are responsible for 30,000 to 50,000 hospitalizations for aseptic meningitis per year in the United States. E30 is one of the most frequently isolated EVs, causing extensive outbreaks in temperate climates in several countries.
Methods
E30 used in this study was isolated from environmental specimens. The virus was confirmed by RT-PCR with specific primers (1). Virus stock was prepared by infecting RD cells in 25 cm2 flasks (Corning Inc. USA). Virus infected and mock infected cells were incubated at 36.5 ºC with 2% MEM. After 48 h of infection, the culture was aliquoted and kept at-80 ºC for further use.
Results
This present work analyzed the E30 genetic diversity in a fragment of 217 nucleotides of the VP1 region, of environmental strains. The environmental samples of echovirus 30 show mutation in the nonfunctional polyprotein protein (Fig. 1). The result suggested that the information may be obtained by analyzing only a part of the VP1 region.
Conclusion
The present study showed that the E30 environmental sample is more divergent to prototype Bastianni strain.
Figure 1
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