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Research
Molecular Characterization of Echovirus 30 Isolated from Environment in North India
Sarika Tiwari
All India Institute of Medical Sciences Jodhpur India
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Echovirus 30 (E30) causes acute aseptic meningitis. Enteroviruses (EVs) are responsible for 30,000 to 50,000 hospitalizations for aseptic meningitis per year in the United States. E30 is one of the most frequently isolated EVs, causing extensive outbreaks in temperate climates in several countries.
Methods
E30 used in this study was isolated from environmental specimens. The virus was confirmed by RT-PCR with specific primers (1). Virus stock was prepared by infecting RD cells in 25 cm2 flasks (Corning Inc. USA). Virus infected and mock infected cells were incubated at 36.5 ºC with 2% MEM. After 48 h of infection, the culture was aliquoted and kept at-80 ºC for further use.
Results
This present work analyzed the E30 genetic diversity in a fragment of 217 nucleotides of the VP1 region, of environmental strains. The environmental samples of echovirus 30 show mutation in the nonfunctional polyprotein protein (Fig. 1). The result suggested that the information may be obtained by analyzing only a part of the VP1 region.
Conclusion
The present study showed that the E30 environmental sample is more divergent to prototype Bastianni strain.
Keywords
Echovirus 30, Aseptic meningitis, Phylogenetic tree, Genetic diversity, VP1 region
Figure 1
Selina Loggins
commented on 20 September, 2020
PLEASE DON’T IGNORE AND READ HOW I WAS CURED FROM HSV-1 AND HSV-2 VIRUS Hello everybody I am here to show appreciation to Doctor Hazim the great herbal doctor that help me get rid of my HSV-1 AND HSV-2 VIRUS, which i was diagnosed with, And after so many research online looking for a cure to my illness which no medical doctor had cure, this left no choice than to share my pains to a friend who had GENITAL HERPES and she directed me to this amazing herbal doctor with great powers who got her cured from GENITAL HERPES to 100% negative and she assured me that Doctor Hazim will help me get rid of my HSV-1 and HSV-2 VIRUS with the help of his herbal remedy and so i decide to give him a try and surprising after taking his herbal remedy for 21 days i was totally cured completely to 100% negative and for 2 months now i have not experienced any symptoms of HSV-1 and HSV-2 virus , i am so happy today to tell you all my story though it's cost me some money but I guarantee you he is one man you can contact for help and he will help you with his great powers. So if you have been looking for cure to whatever illness you are suffering from contact DOCTOR HAZIM by reaching him through his email address ([email protected]) or whatsApp him via his number ( +2349058026857) and you will testify about this great Doctor and i assure you him will never let you down..
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CURRENT STATUS: Under Review
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Posted 18 Sep, 2020
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This preprint is under consideration at Virology Journal. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Molecular Characterization of Echovirus 30 Isolated from Environment in North India
Sarika Tiwari
All India Institute of Medical Sciences Jodhpur India
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 18 Sep, 2020
Community comments: 1
Reviewers invited
Invitations sent on 27 Oct, 2020
Editor assigned
On 17 Sep, 2020
Editor invited
On 16 Sep, 2020
Submission checks complete
On 16 Sep, 2020
First submitted
On 15 Sep, 2020
Metrics
Comments: 1
PDF Downloads: ...
HTML Views: ...
Subject Areas
Virology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Echovirus 30 (E30) causes acute aseptic meningitis. Enteroviruses (EVs) are responsible for 30,000 to 50,000 hospitalizations for aseptic meningitis per year in the United States. E30 is one of the most frequently isolated EVs, causing extensive outbreaks in temperate climates in several countries.
Methods
E30 used in this study was isolated from environmental specimens. The virus was confirmed by RT-PCR with specific primers (1). Virus stock was prepared by infecting RD cells in 25 cm2 flasks (Corning Inc. USA). Virus infected and mock infected cells were incubated at 36.5 ºC with 2% MEM. After 48 h of infection, the culture was aliquoted and kept at-80 ºC for further use.
Results
This present work analyzed the E30 genetic diversity in a fragment of 217 nucleotides of the VP1 region, of environmental strains. The environmental samples of echovirus 30 show mutation in the nonfunctional polyprotein protein (Fig. 1). The result suggested that the information may be obtained by analyzing only a part of the VP1 region.
Conclusion
The present study showed that the E30 environmental sample is more divergent to prototype Bastianni strain.
Figure 1
Selina Loggins
commented on 20 September, 2020
PLEASE DON’T IGNORE AND READ HOW I WAS CURED FROM HSV-1 AND HSV-2 VIRUS Hello everybody I am here to show appreciation to Doctor Hazim the great herbal doctor that help me get rid of my HSV-1 AND HSV-2 VIRUS, which i was diagnosed with, And after so many research online looking for a cure to my illness which no medical doctor had cure, this left no choice than to share my pains to a friend who had GENITAL HERPES and she directed me to this amazing herbal doctor with great powers who got her cured from GENITAL HERPES to 100% negative and she assured me that Doctor Hazim will help me get rid of my HSV-1 and HSV-2 VIRUS with the help of his herbal remedy and so i decide to give him a try and surprising after taking his herbal remedy for 21 days i was totally cured completely to 100% negative and for 2 months now i have not experienced any symptoms of HSV-1 and HSV-2 virus , i am so happy today to tell you all my story though it's cost me some money but I guarantee you he is one man you can contact for help and he will help you with his great powers. So if you have been looking for cure to whatever illness you are suffering from contact DOCTOR HAZIM by reaching him through his email address ([email protected]) or whatsApp him via his number ( +2349058026857) and you will testify about this great Doctor and i assure you him will never let you down..