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Research article
Bo Liu
Fujian Academy of Agricultural Sciences
Corresponding Author
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Background: There is an urgent need to discover alternative antimicrobial agents to control bacterial wilt. The objectives of this study were to report a new lipopeptide-producing biocontrol strain FJAT-46737 and its lipopeptides, and to investigate their antagonistic effects.
Results: Based on the whole genome sequence analysis, the new strain FJAT-46737 was finally identified as Bacillus velezensis and seven gene clusters that responsible for synthesis of bioactive secondary metabolite in the FJAT-46737 were predicted. Antimicrobial results demonstrated that FJAT-46737 exhibited broad-spectrum antimicrobial activities in vitro against bacteria and fungi . The pot experiments showed that the tomato bacterial wilt control efficiencies of the whole cultures, the 2-fold diluted supernatants and the crude lipopeptide of FJAT-46737 were 66.2%, 82.0%, and 96.2%, respectively. The above results suggested that one of the antagonistic mechanisms of FJAT-46737 was secretion of lipopeptides that consisted of iturins, fengycins and surfactins. The crude lipopeptides had significant antagonistic activities on several pathogen in a dosage-dependent manner, and the fengycins confirmed to play a major role in antibacterial abilities in vitro of the lipopeptides against Ralstonia solanacearum . Furthermore, it was found that the rich organic nitrogen sources (especially yeast extracts) in the media were beneficial for FJAT-46737 to produce fengycin and surfactin. The secretion of these two lipopeptides were also adjusted by the culture temperature: the content of the fengycins decreased by 96.6% and that of the surfactins ascended by 59.9% from 20 o C to 40 o C. The temperature of 20 o C~25 o C is the suitable temperature for FJAT-46737 to produce lipopeptide.
Conclusions: The B. velezensis strain FJAT-46737 and its lipopeptides would used as new sources of potential biocontrol agents against plant pathogens, especially the bacterial wilt pathogen R. solanacearum .
Keywords
Bacillus velezensis FJAT-46737, lipopeptides, antagonistic activities, bacterial wilt, fengycin, culture condition
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View the published article at BMC Microbiology.
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Review #2 received
Received 17 May, 2020
Reviewer #2 agreed
On 14 May, 2020
Reviewers invited
Invitations sent on 12 May, 2020
Reviewer #1 agreed
On 12 May, 2020
Editor assigned
On 11 May, 2020
Editor invited
On 10 May, 2020
Submission checks complete
On 10 Nov, 2019
No comments provided
Editorial decision: Major revision
On 03 May, 2020
Review #2 received
Received 02 May, 2020
Review #1 received
Received 28 Apr, 2020
Reviewer #2 agreed
On 10 Apr, 2020
Reviewer #1 agreed
On 09 Apr, 2020
Reviewers invited
Invitations sent on 07 Apr, 2020
Editor assigned
On 06 Apr, 2020
Submission checks complete
On 05 Apr, 2020
Editor invited
On 05 Apr, 2020
No comments provided
Editorial decision: Major revision
On 28 Mar, 2020
Review #2 received
Received 20 Feb, 2020
Review #1 received
Received 20 Feb, 2020
Reviewer #2 agreed
On 08 Feb, 2020
Reviewer #1 agreed
On 07 Feb, 2020
Reviewers invited
Invitations sent on 06 Feb, 2020
Editor assigned
On 05 Feb, 2020
Submission checks complete
On 04 Feb, 2020
Editor invited
On 04 Feb, 2020
Version 2
Posted 19 Dec, 2019
No comments provided
Review #2 received
Received 20 Jan, 2020
Editorial decision: Major revision
On 20 Jan, 2020
Review #1 received
Received 07 Jan, 2020
Reviewer #2 agreed
On 06 Jan, 2020
Reviewer #1 agreed
On 24 Dec, 2019
Reviewers invited
Invitations sent on 21 Dec, 2019
Editor assigned
On 18 Dec, 2019
Submission checks complete
On 17 Dec, 2019
Editor invited
On 17 Dec, 2019
No comments provided
Editorial decision: Major revision
On 01 Dec, 2019
Review #1 received
Received 22 Nov, 2019
Review #2 received
Received 22 Nov, 2019
Reviewer #3 agreed
On 14 Nov, 2019
Reviewer #2 agreed
On 12 Nov, 2019
Editor assigned
On 11 Nov, 2019
Reviewers invited
Invitations sent on 11 Nov, 2019
Reviewer #1 agreed
On 11 Nov, 2019
Submission checks complete
On 10 Nov, 2019
Editor invited
On 10 Nov, 2019
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Applied & Industrial Microbiology
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See the published version of this preprint at BMC Microbiology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Bo Liu
Fujian Academy of Agricultural Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Microbiology.
No community comments so far
Review #2 received
Received 17 May, 2020
Reviewer #2 agreed
On 14 May, 2020
Reviewers invited
Invitations sent on 12 May, 2020
Reviewer #1 agreed
On 12 May, 2020
Editor assigned
On 11 May, 2020
Editor invited
On 10 May, 2020
Submission checks complete
On 10 Nov, 2019
No comments provided
Editorial decision: Major revision
On 03 May, 2020
Review #2 received
Received 02 May, 2020
Review #1 received
Received 28 Apr, 2020
Reviewer #2 agreed
On 10 Apr, 2020
Reviewer #1 agreed
On 09 Apr, 2020
Reviewers invited
Invitations sent on 07 Apr, 2020
Editor assigned
On 06 Apr, 2020
Submission checks complete
On 05 Apr, 2020
Editor invited
On 05 Apr, 2020
No comments provided
Editorial decision: Major revision
On 28 Mar, 2020
Review #2 received
Received 20 Feb, 2020
Review #1 received
Received 20 Feb, 2020
Reviewer #2 agreed
On 08 Feb, 2020
Reviewer #1 agreed
On 07 Feb, 2020
Reviewers invited
Invitations sent on 06 Feb, 2020
Editor assigned
On 05 Feb, 2020
Submission checks complete
On 04 Feb, 2020
Editor invited
On 04 Feb, 2020
Version 2
Posted 19 Dec, 2019
No comments provided
Review #2 received
Received 20 Jan, 2020
Editorial decision: Major revision
On 20 Jan, 2020
Review #1 received
Received 07 Jan, 2020
Reviewer #2 agreed
On 06 Jan, 2020
Reviewer #1 agreed
On 24 Dec, 2019
Reviewers invited
Invitations sent on 21 Dec, 2019
Editor assigned
On 18 Dec, 2019
Submission checks complete
On 17 Dec, 2019
Editor invited
On 17 Dec, 2019
No comments provided
Editorial decision: Major revision
On 01 Dec, 2019
Review #1 received
Received 22 Nov, 2019
Review #2 received
Received 22 Nov, 2019
Reviewer #3 agreed
On 14 Nov, 2019
Reviewer #2 agreed
On 12 Nov, 2019
Editor assigned
On 11 Nov, 2019
Reviewers invited
Invitations sent on 11 Nov, 2019
Reviewer #1 agreed
On 11 Nov, 2019
Submission checks complete
On 10 Nov, 2019
Editor invited
On 10 Nov, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Applied & Industrial Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Microbiology.
Background: There is an urgent need to discover alternative antimicrobial agents to control bacterial wilt. The objectives of this study were to report a new lipopeptide-producing biocontrol strain FJAT-46737 and its lipopeptides, and to investigate their antagonistic effects.
Results: Based on the whole genome sequence analysis, the new strain FJAT-46737 was finally identified as Bacillus velezensis and seven gene clusters that responsible for synthesis of bioactive secondary metabolite in the FJAT-46737 were predicted. Antimicrobial results demonstrated that FJAT-46737 exhibited broad-spectrum antimicrobial activities in vitro against bacteria and fungi . The pot experiments showed that the tomato bacterial wilt control efficiencies of the whole cultures, the 2-fold diluted supernatants and the crude lipopeptide of FJAT-46737 were 66.2%, 82.0%, and 96.2%, respectively. The above results suggested that one of the antagonistic mechanisms of FJAT-46737 was secretion of lipopeptides that consisted of iturins, fengycins and surfactins. The crude lipopeptides had significant antagonistic activities on several pathogen in a dosage-dependent manner, and the fengycins confirmed to play a major role in antibacterial abilities in vitro of the lipopeptides against Ralstonia solanacearum . Furthermore, it was found that the rich organic nitrogen sources (especially yeast extracts) in the media were beneficial for FJAT-46737 to produce fengycin and surfactin. The secretion of these two lipopeptides were also adjusted by the culture temperature: the content of the fengycins decreased by 96.6% and that of the surfactins ascended by 59.9% from 20 o C to 40 o C. The temperature of 20 o C~25 o C is the suitable temperature for FJAT-46737 to produce lipopeptide.
Conclusions: The B. velezensis strain FJAT-46737 and its lipopeptides would used as new sources of potential biocontrol agents against plant pathogens, especially the bacterial wilt pathogen R. solanacearum .
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
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