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Genetic Structure in 21 Remnant Phoebe Sheareri Populations in Southern China: Implications for Genetic Resource Conservation
Yang Wang
State Key Laboratory of Subtropical Silviculture, School of Forestry & Bio-technology, Zhejiang A&F University, Lin’an, Hangzhou, 311300, Zhejiang, China.
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: Phoebe sheareri (Lauraceae) is a valuable and endemic tree species in China, with limited large natural communities remnant. Genetic diversity and differentiation analysis are essential to manage their conservation and utilization. To provide a conservation and utilization strategy of P. sheareri based on sound genetic diversity and differentiation data.
Results: We found medium level of genetic diversity and low inbreeding. Nei's gene diversity and Shannon’s information index value showed medium genetic diversity in nature populations of P. sheareri, which was higher than other two Phoebe species. AMOVA showed the genetic differentiation among populations was significantly, and 21.2% of genetic variation was among populations. Bayesian clustering, obtained with STRUCTURE, grouped the populations into four genetic clusters, whereas UPGMA analysis distinguished three main groups approximately in line with the geographic area of occurrence.
Conclusion: Based on the study results, the establishment of gene conservation units must be considered in nature conserves in order to protect the genetic diversity of the species, and the proposal of sampling strategies for ex situ conservation and reforestation.
Keywords
genetic diversity, Phoebe sheareri, population structure, EST-SSR, protection strategy
Figure 1
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This is a list of supplementary files associated with this preprint. Click to download.
- Fig.S1.tif
Fig.S1 The plotted diagrams of 21 P. sheareri populations diameter-class structure.
- Fig.S2.tif
Fig.S2 Mantel correlations between genetic distance and temperature difference (a) and precipitation difference (b).
- Fig.S3.tif
Fig.S3 Mantel test for the correlation of Nei’s genetic distance with geographical distance (km) and precipitation difference in the western and eastern populations of P. sheareri. The English in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, please see: http://www.textcheck.com/certificate/SgDzXS
- TableS1.doc
Table S1 Characteristics of 32 pairs of microsatellite makers used for the analysis of genetic diversity and structure of 21 natural P. sheareri populations.
- TableS2.doc
Table S2 Polymorphism analysis of SSR primers in 32 SSR loci.
- TableS3.doc
Table S3 Coefficient of genetic differentiation and gene flow in populations of P. sheareri.
- TableS4.doc
Table S4 Genetic distance and genetic identity among 21 natural populations of P. sheareri.
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Research
Genetic Structure in 21 Remnant Phoebe Sheareri Populations in Southern China: Implications for Genetic Resource Conservation
Yang Wang
State Key Laboratory of Subtropical Silviculture, School of Forestry & Bio-technology, Zhejiang A&F University, Lin’an, Hangzhou, 311300, Zhejiang, China.
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 22 Sep, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Forestry
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Phoebe sheareri (Lauraceae) is a valuable and endemic tree species in China, with limited large natural communities remnant. Genetic diversity and differentiation analysis are essential to manage their conservation and utilization. To provide a conservation and utilization strategy of P. sheareri based on sound genetic diversity and differentiation data.
Results: We found medium level of genetic diversity and low inbreeding. Nei's gene diversity and Shannon’s information index value showed medium genetic diversity in nature populations of P. sheareri, which was higher than other two Phoebe species. AMOVA showed the genetic differentiation among populations was significantly, and 21.2% of genetic variation was among populations. Bayesian clustering, obtained with STRUCTURE, grouped the populations into four genetic clusters, whereas UPGMA analysis distinguished three main groups approximately in line with the geographic area of occurrence.
Conclusion: Based on the study results, the establishment of gene conservation units must be considered in nature conserves in order to protect the genetic diversity of the species, and the proposal of sampling strategies for ex situ conservation and reforestation.
Figure 1
Figure 2
Figure 3
Figure 4