Genetic Structure in 21 Remnant Phoebe Sheareri Populations in Southern China: Implications for Genetic Resource Conservation
Background: Phoebe sheareri (Lauraceae) is a valuable and endemic tree species in China, with limited large natural communities remnant. Genetic diversity and differentiation analysis are essential to manage their conservation and utilization. To provide a conservation and utilization strategy of P. sheareri based on sound genetic diversity and differentiation data.
Results: We found medium level of genetic diversity and low inbreeding. Nei's gene diversity and Shannon’s information index value showed medium genetic diversity in nature populations of P. sheareri, which was higher than other two Phoebe species. AMOVA showed the genetic differentiation among populations was significantly, and 21.2% of genetic variation was among populations. Bayesian clustering, obtained with STRUCTURE, grouped the populations into four genetic clusters, whereas UPGMA analysis distinguished three main groups approximately in line with the geographic area of occurrence.
Conclusion: Based on the study results, the establishment of gene conservation units must be considered in nature conserves in order to protect the genetic diversity of the species, and the proposal of sampling strategies for ex situ conservation and reforestation.
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Fig.S1 The plotted diagrams of 21 P. sheareri populations diameter-class structure.
Fig.S2 Mantel correlations between genetic distance and temperature difference (a) and precipitation difference (b).
Fig.S3 Mantel test for the correlation of Nei’s genetic distance with geographical distance (km) and precipitation difference in the western and eastern populations of P. sheareri. The English in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, please see: http://www.textcheck.com/certificate/SgDzXS
Table S1 Characteristics of 32 pairs of microsatellite makers used for the analysis of genetic diversity and structure of 21 natural P. sheareri populations.
Table S2 Polymorphism analysis of SSR primers in 32 SSR loci.
Table S3 Coefficient of genetic differentiation and gene flow in populations of P. sheareri.
Table S4 Genetic distance and genetic identity among 21 natural populations of P. sheareri.
Posted 22 Sep, 2020
Genetic Structure in 21 Remnant Phoebe Sheareri Populations in Southern China: Implications for Genetic Resource Conservation
Posted 22 Sep, 2020
Background: Phoebe sheareri (Lauraceae) is a valuable and endemic tree species in China, with limited large natural communities remnant. Genetic diversity and differentiation analysis are essential to manage their conservation and utilization. To provide a conservation and utilization strategy of P. sheareri based on sound genetic diversity and differentiation data.
Results: We found medium level of genetic diversity and low inbreeding. Nei's gene diversity and Shannon’s information index value showed medium genetic diversity in nature populations of P. sheareri, which was higher than other two Phoebe species. AMOVA showed the genetic differentiation among populations was significantly, and 21.2% of genetic variation was among populations. Bayesian clustering, obtained with STRUCTURE, grouped the populations into four genetic clusters, whereas UPGMA analysis distinguished three main groups approximately in line with the geographic area of occurrence.
Conclusion: Based on the study results, the establishment of gene conservation units must be considered in nature conserves in order to protect the genetic diversity of the species, and the proposal of sampling strategies for ex situ conservation and reforestation.
Figure 1
Figure 2
Figure 3
Figure 4