Non-small cell lung cancer (NSCLC) is a part of the most common cancers in the world. A lot of efforts have been made to clarify the etiology of non-small cell lung cancer, but the molecular mechanism of non-small cell lung cancer is still unclear.
In order to identify candidate genes in the occurrence and progression of non-small cell lung cancer, GSE19804 GSE118370 GSE19188 GSE27262 and GSE33532 microarray data sets were downloaded from the Gene Expression General (GEO) database. Identify differentially expressed genes (DEGS) and perform functional enrichment analysis. The protein-protein interaction network (PPI) was constructed, and the module analysis was performed using STRING and Cytoscape. A total of 562 DEGS were identified, consisting of 98 downregulated genes and 464 upregulated genes. Abundant functions and pathways of DEGS include p53 signaling pathway, Cell adhesion molecules,Leukocyte transendothelial migration,Vascular smooth muscle contraction,Complement and coagulation cascades and Axon guidance. Tumor immunity was assessed to investigate the functions of hub genes.
Totally 562 genes were found to be dysregulated. 12 genes were considered to be the hub genes. NUF2 was considered as the potential immunotherapeutic targets with future clinical significance.12 hub genes were detected, and biological process analysis showed that these genes are mainly enriched in p53 signaling pathway,Progesterone-mediated oocyte maturation,Cell cycle, Oocyte meiosis and Cellular senescence Survival analysis shows that NUF2 may be linked to the occurrence, invasion or recurrence of non-small cell lung cancer.
NUF2 genes discovered in this study help us include the molecular mechanisms of the occurrence and progression of non-small cell lung cancer, and provide candidate targets for the diagnosis and treatment of non-small cell lung cancer.