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In vivo Antimalarial Activity of the Hydroalcoholic Extract of Kniphofia foliosa Hochst and Its Constituents
Kaleab Asres
Addis Ababa University College of Health Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1212-8688
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This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Malaria Journal.
Background: Kniphofia foliosa Hochst is endemic to Ethiopian highlands, where its rhizomes are traditionally used for the treatment of malaria, abdominal cramps and wound healing. As a continuation of our search for antimalarial compounds from Ethiopian medicinal plants, we have tested the 80% methanol extract of K. foliosa rhizomes and its constituents against Plasmodium berghei in mice.
Methods: Isolation was carried out using column and preparative thin layer chromatography (PTLC). The chemical structures of the compounds were elucidated by spectroscopic methods (ESI-MS, 1D and 2D-NMR). Peters’ 4-day suppressive test against P. berghei in mice was utilized for in vivo antimalarial evaluation of the test substances.
Results: Three compounds, namely knipholone, dianellin, and 12-hydroxypentadec-9-en-1-yl methyl phthalate (HPMP) were isolated and characterized from the 80% methanolic extract of K. foliosa rhizomes. The hydroalcoholic extract (400 mg/kg) and knipholone (200 mg/kg) showed the highest activity with chemosuppression values of 61.52 and 60.16%, respectively. From the dose-response plot, the median effective (ED50) doses of knipholone and dianellin were determined to be 81.25 and 92.31 mg/kg, respectively. Molecular docking study revealed that knipholone had a strong binding affinity to Plasmodium falciparum l-lactate dehydrogenase (pfLDH) target.
Conclusion: Results of the current study support the traditional use of the plant for the treatment of malaria.
Keywords
Kniphofia foliosa, antimalarial activity, phenolic fractions, knipholone, dianellin
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CURRENT STATUS: Published
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Posted 30 Nov, 2020
Editorial decision: Minor Revision
On 30 Nov, 2020
Editor assigned
On 22 Nov, 2020
Submission checks complete
On 22 Nov, 2020
Editor invited
On 22 Nov, 2020
This version was not preprinted
Version 1
Posted 23 Sep, 2020
Published
On 01 Jan, 2021
No community comments so far
Review #3 received
Received 25 Oct, 2020
Editorial decision: Major Revision
On 25 Oct, 2020
Review #2 received
Received 19 Oct, 2020
Review #1 received
Received 16 Oct, 2020
Reviewer #3 agreed
On 29 Sep, 2020
Reviewers invited
Invitations sent on 28 Sep, 2020
Reviewer #1 agreed
On 28 Sep, 2020
Reviewer #2 agreed
On 28 Sep, 2020
Editor assigned
On 19 Sep, 2020
Submission checks complete
On 19 Sep, 2020
Editor invited
On 18 Sep, 2020
First submitted
On 16 Sep, 2020
Metrics
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PDF Downloads: ...
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Subject Areas
Infectious Diseases
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This preprint is under consideration at Malaria Journal. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
In vivo Antimalarial Activity of the Hydroalcoholic Extract of Kniphofia foliosa Hochst and Its Constituents
Kaleab Asres
Addis Ababa University College of Health Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1212-8688
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version (no preprint)
Posted 30 Nov, 2020
Editorial decision: Minor Revision
On 30 Nov, 2020
Editor assigned
On 22 Nov, 2020
Submission checks complete
On 22 Nov, 2020
Editor invited
On 22 Nov, 2020
This version was not preprinted
Version 1
Posted 23 Sep, 2020
Published
On 01 Jan, 2021
No community comments so far
Review #3 received
Received 25 Oct, 2020
Editorial decision: Major Revision
On 25 Oct, 2020
Review #2 received
Received 19 Oct, 2020
Review #1 received
Received 16 Oct, 2020
Reviewer #3 agreed
On 29 Sep, 2020
Reviewers invited
Invitations sent on 28 Sep, 2020
Reviewer #1 agreed
On 28 Sep, 2020
Reviewer #2 agreed
On 28 Sep, 2020
Editor assigned
On 19 Sep, 2020
Submission checks complete
On 19 Sep, 2020
Editor invited
On 18 Sep, 2020
First submitted
On 16 Sep, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Infectious Diseases
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Malaria Journal.
Background: Kniphofia foliosa Hochst is endemic to Ethiopian highlands, where its rhizomes are traditionally used for the treatment of malaria, abdominal cramps and wound healing. As a continuation of our search for antimalarial compounds from Ethiopian medicinal plants, we have tested the 80% methanol extract of K. foliosa rhizomes and its constituents against Plasmodium berghei in mice.
Methods: Isolation was carried out using column and preparative thin layer chromatography (PTLC). The chemical structures of the compounds were elucidated by spectroscopic methods (ESI-MS, 1D and 2D-NMR). Peters’ 4-day suppressive test against P. berghei in mice was utilized for in vivo antimalarial evaluation of the test substances.
Results: Three compounds, namely knipholone, dianellin, and 12-hydroxypentadec-9-en-1-yl methyl phthalate (HPMP) were isolated and characterized from the 80% methanolic extract of K. foliosa rhizomes. The hydroalcoholic extract (400 mg/kg) and knipholone (200 mg/kg) showed the highest activity with chemosuppression values of 61.52 and 60.16%, respectively. From the dose-response plot, the median effective (ED50) doses of knipholone and dianellin were determined to be 81.25 and 92.31 mg/kg, respectively. Molecular docking study revealed that knipholone had a strong binding affinity to Plasmodium falciparum l-lactate dehydrogenase (pfLDH) target.
Conclusion: Results of the current study support the traditional use of the plant for the treatment of malaria.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5