This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Primary research
Zhisen Shen
Ningbo Medical Treatment Centre Li Huili Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6660-0488
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Transfer RNA (tRNA) is a ubiquitous RNA, present in all life forms and considered a housekeeping gene with limited regulatory function. Recent studies have shown that tRNA is dysregulated and involved in the pathogenic process of cancer.
Methods: Quantitative reverse transcription-polymerase chain reaction detected the relative expression levels of tRNAIniCAT in paired LSCC tissues and paracancerous tissues and preoperative, postoperative, and healthy human plasma. We analyzed the relationship between its expression level and the clinicopathological features of patients. We also established the receiver operating characteristic curve and predicted its diagnostic value. High expression of tRNAIniCAT was detected in the LSCC cell line. The proliferation, apoptosis, and cycle of cells were analyzed. High expression of tRNAIniCAT was detected in mice with transplanted tumors. The tumor weight and volume in mice were measured. The transplanted tumor was used for pathological analysis and transmission electron microscope observation.
Results: tRNAIniCAT was down-regulated in LSCC tissues and plasma. The area under the plasma receiver operating characteristic curve was 0.808. High expression of tRNAIniCAT in the laryngeal carcinoma cell line inhibited cell proliferation and promoted apoptosis. High expression of tRNAIniCAT in transplanted tumor in nude mice inhibited the growth of the transplanted tumor.
Conclusion: tRNAIniCAT acts as a tumor Inhibitor in LSCC, inhibits cell proliferation, and promotes apoptosis in vitro and in vivo. tRNAIniCAT may be used as a new biomarker for the early diagnosis of LSCC.
Keywords
tRNA, tRNAIniCAT, biomarkers, laryngeal squamous cell carcinoma, diagnosis
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Primary research
Zhisen Shen
Ningbo Medical Treatment Centre Li Huili Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6660-0488
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 21 Sep, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Transfer RNA (tRNA) is a ubiquitous RNA, present in all life forms and considered a housekeeping gene with limited regulatory function. Recent studies have shown that tRNA is dysregulated and involved in the pathogenic process of cancer.
Methods: Quantitative reverse transcription-polymerase chain reaction detected the relative expression levels of tRNAIniCAT in paired LSCC tissues and paracancerous tissues and preoperative, postoperative, and healthy human plasma. We analyzed the relationship between its expression level and the clinicopathological features of patients. We also established the receiver operating characteristic curve and predicted its diagnostic value. High expression of tRNAIniCAT was detected in the LSCC cell line. The proliferation, apoptosis, and cycle of cells were analyzed. High expression of tRNAIniCAT was detected in mice with transplanted tumors. The tumor weight and volume in mice were measured. The transplanted tumor was used for pathological analysis and transmission electron microscope observation.
Results: tRNAIniCAT was down-regulated in LSCC tissues and plasma. The area under the plasma receiver operating characteristic curve was 0.808. High expression of tRNAIniCAT in the laryngeal carcinoma cell line inhibited cell proliferation and promoted apoptosis. High expression of tRNAIniCAT in transplanted tumor in nude mice inhibited the growth of the transplanted tumor.
Conclusion: tRNAIniCAT acts as a tumor Inhibitor in LSCC, inhibits cell proliferation, and promotes apoptosis in vitro and in vivo. tRNAIniCAT may be used as a new biomarker for the early diagnosis of LSCC.
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