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Protein Regulation Strategies of Mouse Spleen in Response to Babesia Microti Infection
Jing-Ze Liu
Hebei Normal University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Babesia is a protozoan parasite in red blood cells of some vertebrates. Some species of Babesia can cause zoonoses and cause great harm. As the largest immune organ in mammals, the spleen plays an important role in defending against Babesia infection. When infected with Babesia, the spleen is seriously injured, but it still actively initiates immunomodulatory responses.
Methods
In order to explore the molecular mechanisms underlying the immune regulation and self-repair of the spleen in response to infection, this study used data-independent acquisition (DIA) quantitative proteomics to analyse changes in expression levels of global proteins and changes in phosphorylation modification in spleen tissue after Babesia microti infection in mice.
Results
After the mice were infected with B. microti, their spleen were seriously damaged.Using bioinfor-matics methods to analyze the dynamic changes of a large number of proteins, we found that spleen still initiated immune response to deal with the infection, in which immune-related proteins played an important role, including CTSD, IFI44, ILF2, ILF, and STAT5A. In addition, some proteins related to iron metabolism were also involved in the repair of spleen against B. microti infection, including serotransferrin, lactoferrin, TfR1, and GCL. At the same time, the expression and phosphorylation of proteins related to the growth and development of the spleen also changed, including PKC-δ and MAPK3/1, Grb2, and PAK2.
Conclusions
Immune-related proteins, iron metabolism-related proteins and growth and development-related proteins play an important important role in the regulation of spleen injury and maintenance of homeostasis. This study will provide important bases for the diagnosis and treatment of babesiosis.
Keywords
Babesia microti, spleen, quantitative proteomics, phosphorylation, immune
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On 13 Dec, 2020
Review #1 received
Received 13 Dec, 2020
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Invitations sent on 10 Dec, 2020
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On 08 Dec, 2020
Editor invited
On 08 Dec, 2020
Version 1
Posted 25 Sep, 2020
No community comments so far
Review #4 received
Received 20 Oct, 2020
Editorial decision: Major Revision
On 20 Oct, 2020
Review #2 received
Received 06 Oct, 2020
Review #1 received
Received 06 Oct, 2020
Review #3 received
Received 30 Sep, 2020
Reviewer #4 agreed
On 29 Sep, 2020
Reviewer #3 agreed
On 28 Sep, 2020
Reviewer #1 agreed
On 25 Sep, 2020
Reviewer #2 agreed
On 25 Sep, 2020
Editor assigned
On 22 Sep, 2020
Reviewers invited
Invitations sent on 22 Sep, 2020
First submitted
On 21 Sep, 2020
Submission checks complete
On 21 Sep, 2020
Editor invited
On 21 Sep, 2020
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Subject Areas
Parasitology
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This preprint is under consideration at Parasites & Vectors. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Protein Regulation Strategies of Mouse Spleen in Response to Babesia Microti Infection
Jing-Ze Liu
Hebei Normal University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Review #3 received
Received 21 Dec, 2020
Reviewer #4 agreed
On 19 Dec, 2020
Review #4 received
Received 19 Dec, 2020
Reviewer #3 agreed
On 15 Dec, 2020
Review #2 received
Received 14 Dec, 2020
Reviewer #2 agreed
On 13 Dec, 2020
Reviewer #1 agreed
On 13 Dec, 2020
Review #1 received
Received 13 Dec, 2020
Reviewers invited
Invitations sent on 10 Dec, 2020
Editor assigned
On 08 Dec, 2020
Submission checks complete
On 08 Dec, 2020
Editor invited
On 08 Dec, 2020
Version 1
Posted 25 Sep, 2020
No community comments so far
Review #4 received
Received 20 Oct, 2020
Editorial decision: Major Revision
On 20 Oct, 2020
Review #2 received
Received 06 Oct, 2020
Review #1 received
Received 06 Oct, 2020
Review #3 received
Received 30 Sep, 2020
Reviewer #4 agreed
On 29 Sep, 2020
Reviewer #3 agreed
On 28 Sep, 2020
Reviewer #1 agreed
On 25 Sep, 2020
Reviewer #2 agreed
On 25 Sep, 2020
Editor assigned
On 22 Sep, 2020
Reviewers invited
Invitations sent on 22 Sep, 2020
First submitted
On 21 Sep, 2020
Submission checks complete
On 21 Sep, 2020
Editor invited
On 21 Sep, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Babesia is a protozoan parasite in red blood cells of some vertebrates. Some species of Babesia can cause zoonoses and cause great harm. As the largest immune organ in mammals, the spleen plays an important role in defending against Babesia infection. When infected with Babesia, the spleen is seriously injured, but it still actively initiates immunomodulatory responses.
Methods
In order to explore the molecular mechanisms underlying the immune regulation and self-repair of the spleen in response to infection, this study used data-independent acquisition (DIA) quantitative proteomics to analyse changes in expression levels of global proteins and changes in phosphorylation modification in spleen tissue after Babesia microti infection in mice.
Results
After the mice were infected with B. microti, their spleen were seriously damaged.Using bioinfor-matics methods to analyze the dynamic changes of a large number of proteins, we found that spleen still initiated immune response to deal with the infection, in which immune-related proteins played an important role, including CTSD, IFI44, ILF2, ILF, and STAT5A. In addition, some proteins related to iron metabolism were also involved in the repair of spleen against B. microti infection, including serotransferrin, lactoferrin, TfR1, and GCL. At the same time, the expression and phosphorylation of proteins related to the growth and development of the spleen also changed, including PKC-δ and MAPK3/1, Grb2, and PAK2.
Conclusions
Immune-related proteins, iron metabolism-related proteins and growth and development-related proteins play an important important role in the regulation of spleen injury and maintenance of homeostasis. This study will provide important bases for the diagnosis and treatment of babesiosis.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8