In this study, a novel five degrees-of-freedom nonlinear dynamic model of linear guide slide platform is proposed, the model considers the effect of assembly error. The assembly errors are modeled as five types including: straightness assembly errors along x and y axes and rotation assembly error around x, y and z axes. The assembly error induced displacement of platform is considered. The restoring force and moment of the carriages is equal to zero when no dynamic or static load applied on the platform with considering the influence of assembly error. The influence of assembly error on the static and dynamic characteristics are investigated. The results indicate that the assembly error can cause uneven load distribution, change the dynamics of the system. In addition, the stability of the system cannot be improved by simply increasing the preload. Moreover, a series of experiments are conducted on a specialized platform to estimate the parameters of the system and verify the proposed model.