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Integrated proteomic and metabolomic analysis to study the effects of spaceflight on Candida albicans
Yongzhi Li
Astronaut Center of China
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0007-0514
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomics.
Background: Candida albicans is an opportunistic pathogenic yeast, which could become pathogenic in various stressful environmental factors including the spaceflight environment. In this study, we aim to explore the phenotypic changes and possible mechanisms of C. albicans after exposure to spaceflight conditions. Results: The effect of C. albicans after carried on the "SJ-10" satellite for 12 days was evaluated by proliferation, morphology, environmental resistance and virulence experiment. The result showed that the proliferation rate, biofilm formation, antioxidant capacity, cytotoxicity and filamentous morphology of C. albicans were increased in the spaceflight group compared to the control group. Proteomics and metabolomics technologies were used to analyze the profiles of proteins and metabolites in C. albicans under spaceflight conditions. Proteomic analysis identified 548 up-regulated proteins involved in the ribosome, DNA replication, base excision repair and sulfur metabolism in the spaceflight group. Moreover, 332 down-regulated proteins related to metabolic processes were observed. The metabolomic analysis found five differentially expressed metabolites. The combined analysis of proteomic and metabolomic revealed the accumulation of cysteine and methionine in C. albicans after spaceflight. Conclusions: Mechanisms that could explain the results in the phenotypic experiment of C. albicans were found through proteomic and metabolomic analysis. And our data provide an important basis for the assessment of the risk that C. albicans could cause under spaceflight environment.
Keywords
Spaceflight, Metabolomics, Proteomics, Candida albicans
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CURRENT STATUS: Published
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Posted 14 Jan, 2020
Published
On 17 Jan, 2020
No community comments so far
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On 10 Jan, 2020
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On 09 Jan, 2020
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Editorial decision: Minor revision
On 06 Jan, 2020
Editor assigned
On 02 Jan, 2020
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On 01 Jan, 2020
Editor invited
On 01 Jan, 2020
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Editorial decision: Minor revision
On 26 Dec, 2019
Review #1 received
Received 25 Dec, 2019
Review #2 received
Received 16 Dec, 2019
Reviewer #2 agreed
On 15 Dec, 2019
Reviewers invited
Invitations sent on 13 Dec, 2019
Reviewer #1 agreed
On 13 Dec, 2019
Editor assigned
On 12 Dec, 2019
Submission checks complete
On 11 Dec, 2019
Editor invited
On 11 Dec, 2019
No comments provided
Review #2 received
Received 08 Dec, 2019
Editorial decision: Minor revision
On 08 Dec, 2019
Review #1 received
Received 30 Nov, 2019
Reviewer #2 agreed
On 26 Nov, 2019
Reviewers invited
Invitations sent on 19 Nov, 2019
Reviewer #1 agreed
On 19 Nov, 2019
Editor assigned
On 13 Nov, 2019
Submission checks complete
On 12 Nov, 2019
Editor invited
On 12 Nov, 2019
First submitted
On 11 Nov, 2019
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Subject Areas
Epigenetics & Genomics
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This preprint is under consideration at BMC Genomics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Integrated proteomic and metabolomic analysis to study the effects of spaceflight on Candida albicans
Yongzhi Li
Astronaut Center of China
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0007-0514
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 4
Posted 14 Jan, 2020
Published
On 17 Jan, 2020
No community comments so far
Submission checks complete
On 10 Jan, 2020
Editorial decision: Accept
On 09 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 06 Jan, 2020
Editor assigned
On 02 Jan, 2020
Submission checks complete
On 01 Jan, 2020
Editor invited
On 01 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 26 Dec, 2019
Review #1 received
Received 25 Dec, 2019
Review #2 received
Received 16 Dec, 2019
Reviewer #2 agreed
On 15 Dec, 2019
Reviewers invited
Invitations sent on 13 Dec, 2019
Reviewer #1 agreed
On 13 Dec, 2019
Editor assigned
On 12 Dec, 2019
Submission checks complete
On 11 Dec, 2019
Editor invited
On 11 Dec, 2019
No comments provided
Review #2 received
Received 08 Dec, 2019
Editorial decision: Minor revision
On 08 Dec, 2019
Review #1 received
Received 30 Nov, 2019
Reviewer #2 agreed
On 26 Nov, 2019
Reviewers invited
Invitations sent on 19 Nov, 2019
Reviewer #1 agreed
On 19 Nov, 2019
Editor assigned
On 13 Nov, 2019
Submission checks complete
On 12 Nov, 2019
Editor invited
On 12 Nov, 2019
First submitted
On 11 Nov, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomics.
Background: Candida albicans is an opportunistic pathogenic yeast, which could become pathogenic in various stressful environmental factors including the spaceflight environment. In this study, we aim to explore the phenotypic changes and possible mechanisms of C. albicans after exposure to spaceflight conditions. Results: The effect of C. albicans after carried on the "SJ-10" satellite for 12 days was evaluated by proliferation, morphology, environmental resistance and virulence experiment. The result showed that the proliferation rate, biofilm formation, antioxidant capacity, cytotoxicity and filamentous morphology of C. albicans were increased in the spaceflight group compared to the control group. Proteomics and metabolomics technologies were used to analyze the profiles of proteins and metabolites in C. albicans under spaceflight conditions. Proteomic analysis identified 548 up-regulated proteins involved in the ribosome, DNA replication, base excision repair and sulfur metabolism in the spaceflight group. Moreover, 332 down-regulated proteins related to metabolic processes were observed. The metabolomic analysis found five differentially expressed metabolites. The combined analysis of proteomic and metabolomic revealed the accumulation of cysteine and methionine in C. albicans after spaceflight. Conclusions: Mechanisms that could explain the results in the phenotypic experiment of C. albicans were found through proteomic and metabolomic analysis. And our data provide an important basis for the assessment of the risk that C. albicans could cause under spaceflight environment.
Figure 1
Figure 2
Figure 3