Distribution and Molecular Analysis of Subtilase Cytotoxin Gene (subAB) Variants in Shiga Toxin-Producing Escherichia Coli (STEC) Isolated From Different Sources in Iran

doi:10.21203/rs.3.rs-853144/v1

Background

Subtilase is a potent cytotoxin that was first described in O113:H21 strain in Australia as a plasmid- encoded cytotoxin (subAB1). Subsequently, chromosomal variants including subAB2-1, subAB2-2, and subAB2-3 were described.

Results

In the present study a collection of 101 archived STEC strains isolated from various sources in Iran (2009–2016) were analyzed for the detection of different genes encoding the subtilase variants, plasmidic and chromosomal virulence genes, together with the phylogroup and serogroups. Overall, 57 isolates (56.4%) carried at least one variant of subAB. Most strains from small ruminants including 93% of sheep and 96% of caprine isolates carried at least one chromosomally encoded variant (subAB-2-1 and/or subAb2-2). In contrast, 12 cattle isolates (24%) only harbored the plasmid encoded variant (subAB1). STEC strains from other sources including deer, pony and humans were positive for subAB-2-1 and/or subAb2-2. Concerning the virulence markers, some strains showed an association with hosts the bacteria were isolated from. In particular, tia was associated with sheep, goats and pony isolates and astA gene was present in deer, pony and goats and terD was only found in deer and pony isolates. Only cattle STEC carried espP and epeA, the important markers of pO113 plasmid. Some genes were widespread among strain of various sources like ehly, iha and lpf^O113 and some genes were not detected such as efa1, toxB and katP. Most strains belonged to phylogenetic group B1 (89.47%), but five strains from cattle, deer, pony and a goat were assigned to A phylogroup. Most cattle strains belonged to O113, while O5 was just detected in ovine isolates, and O128 and O113 were present in caprine strains.

Conclusions

the present study reveals the presence of potentially pathogenic genotypes among LEE-negative isolates and some host specificity related to subtilase variants and other virulence markers that may aid in source tracking of STEC during outbreak investigations.

General Microbiology

Subtilase variants

LEE-negative

STEC

animals

source tracking

Shiga toxin- producing E. coli (STEC) is considered as one of the most important food-borne pathogens worldwide causing diseases in humans with different severity such as diarrhea, hemorrhagic colitis (HC), and some fatal conditions such as hemolytic uremic syndrome (HUS).

The early reported large outbreaks were due to O157:H7 E. coli, which harbor sets of important virulence markers beside the Shiga toxin (s) coding genes, such as locus of entrocyte effacement (LEE) and Enterohemorrhagic E. coli hemolysin (ehly) (Gyles 2006). Later on, it become evident that STEC belonging to other serogroups and showing different genotypes, can also cause severe infections and outbreaks. For example, some strains isolated from HUS are negative for the LEE locus and belong to diverse serogroups such as O55, O73, O91, O104, O113, O128, O145, O163, O178 [1, 2]. Most of our knowledge on pathogenesis of STEC infection derives from the study of the strains belonging to five serogroups such as O157, O111, O103, O145 and O26, described as belonging to the seropathotypes A and B [3], however, more recent studies are highlighting the particular importance of LEE-negative strains and emerging seropathotypes [4–6]. Notably, one of the largest and most severe HUS outbreaks was attributed to O104: H4, a hybrid LEE-negative Stx2-producing strain with enteroaggregative genomic backbone [7].

Among the virulence factors of highly pathogenic LEE-negative STEC, subtilase cytotoxin was described to be one of the major player as recent studies suggest [8, 9]. Subtilase is a potent AB5 toxin showing high cytotoxicity to Vero cells and lethality when injected intraperitoneally to mice [10]. Wild type SubAB encoding strain provoked cytotoxic effect almost similar to the highly pathogenic O157:H7 strain (EDL933) [11]. Additionally, besides damaging renal epithelial cells, in a mice experimental model it also induces multi-organ systemic response very similar to HUS pathogenesis [9].

Subtilase, was first described in 2004 in O113:H21 strain (98NK2 ) isolated during a HUS outbreak in southern Australia [10]. This novel toxin was first described to be encoded by a operon comprising two components of subA and subB co-transcribed from genes located on pO113 transmissible megaplasmid which subsequently named subAB1 [10, 12]. Other studies demonstrated the presence of chromosomally encoded variants in small ruminants and other STEC strains and named subAB2-1 and subAB2-2 [13, 14]. The subAB2-1 is carried on a pathogenicity island SE-PAI and in most instances was linked to tia gene which encodes invasion protein first reported in enterotoxigenic E. coli. The subAB2-2 is adjacent to outer membrane efflux protein locus (OEP); moreover, a novel variant was also discovered as subAB2-3 in deer STEC (Strain 48) in 2014 [13–15].

Many studies in Iran showed that non-O157 STEC strains are widely distributed in food producing animals. We recently demonstrated the virulence properties of non-O157 STEC in cattle and small ruminants in Iran [16, 17]. As our data showed so far, the prevalence of LEE-negative non-O157 strains are quite high; therefore, we aimed to investigate the most important virulence determinants in such strains for the first time. For this purpose, we examined the presence of subAB genes in a collection of STEC strains isolated from different sources during 2007 to 2016 then we determined the allelic variants, virulence determinants, serogroups and phylogroups of the subtilase-producing STEC in Iran.

E. coli strains

A total of 101 STEC strains isolated from different sources in three veterinary institutions in Iran during the period from 2007 to 2016 were selected for this study. To test the purity of the isolates, they were sub-cultured on MacConkey agar and a single colony was used in subsequent analysis. The presence of Shiga toxin genes (stx), was confirmed using a multiplex-PCR targeting stx1, stx2, eae, and ehly as described previously [18]. The isolates were obtained from cattle (n=50), goats (n=25), sheep (n=15), wild captive animals (n=8) and humans (n=3) as shown in Table 1.

PCR detection of subAB genes and determination of the allelic variants

The STEC isolates were first subjected to a PCR assay recognizing different chromosomal and/or plasmid encoded Subtilase variants. Then, the subAB+ isolates were analyzed by PCR to discriminate allelic variants of the Subtilase gene. The subAB1 and subAB2-2 variants were detected as described by Funk et al. (2013), and the subAB2-1 was identified as described by Michelacci et al. (2013). For the detection of the novel subAB2-3 variant, a pair of primers was designed according to the published sequence of this variant (accession no. JPQG00000000); primers were also tested in silico against the deposited sequences containing this variant (http://insilico.ehu.es/PCR/). The primers were SubB2-3 (5’-AACGCCTGAAAACATGCCAT-3’), and JD73R (5’-CGCTATTCTCGCAGGTACAG-3’) amplifying a 2037 bp fragment of the novel variant and the adjacent hypothetical gene. The condition for amplification of subAb2-3 consisted of 94 °C (60s), 55 °C (60s), and 72 °C (120s) and repeated for 35 cycles.

Virulence genes and genetic determinants

All subAB+ strains were subjected to PCR analysis for various virulence genes. The presence of some plasmid encoded genes such as saa, espP, epeA, toxB, and katP were investigated as described previously [19]. Presence of other chromosomally encoded virulence/genetic determinants including astA , cdt, iha, efa1, lpf O113 and terD were also tested by PCR as described before [19–21].

Phylogenetic groups

All strains carrying subAB were subjected to the updated protocol for E. coli phylogenetic grouping. First, the strains were tested by a quadruplex-PCR, and if the strain was not assigned to a particular phylogroup, complementary PCRs were conducted as described before [22].

Molecular serotyping

All strains were tested for eight pathogenic STEC serogroups including O26, O45, O103, O111, O113, O121, O145 and O157 using a multiplex-PCR as described previously [23]. If the strains were negative for the top eight serogroups, the isolates were additionally tested for some other prevalent serogroups mostly associated with LEE-negative and subAB- encoding strains including O5, O91, O104, O113, and O128. The primers and PCRs were used as described previously [24, 25].

Screening PCR and allelic variants of subAB

In total, 57 of the 101 STEC tested (56.4%), yielded the specific amplicon for subAB. All positive isolates were typically the LEE-negative strains (Table 1). Most STEC from small ruminants including 93% of strains from sheep and 96% from goats carried at least one chromosomally encoded subAB variant; in fact, with two exceptions all carried both subAB2-1 and subAB2-2. In contrast, of 50 cattle STEC isolates, only 12 (24%) carried the plasmid encoded variant (sub AB1). As presented in Table 2, four strains from deer and pony and three from diarrheic children were positive for subAB2-1 and/or subAB2-2. None of the studied isolates yielded the specific amplicon for subAB2-3.

Table 1 The E. coli isolates from different sources and distribution of major virulence genes with regard to subtilase possession

No. of Isolates	Virulence genes				No. of isolates (No. of subAB+)
No. of Isolates	stx1	stx2	eae	ehly	Sheep (n=15)	Goats (n=25)	Cattle (n=50)	Other species (n=11)
6	+					5 (3)	1
7		+					7
1	+	+					1
23	+	+		+	3 (3)	9 (9)	8	3^b (3)
17		+		+		1	15 (12)	1^c (1)
26	+			+	11 (11)	11 (11)	1	3^d (3)
13	+		+	+			13
6		+	+	+			2	4^e
1	+	+	+	+			1
1	+		+				1
28		+^a	+
Total Positive (%)					14 (93.3)	24 (92.0)	12 (24.0)	7 (63.6)

^astx2f+

^bPersian follow deer (n=2), Caspian pony (n=1)

^cPersian follow deer

^dDiarrheic children

^eMacaca mulatta

Table 2 Distribution of Subtilase variants, Shiga toxin gene(s), O-serogroups and phylogenetic groups among isolates from various sources

Genetic traits		Hosts (no. of subAB+ isolates)				Total (57)
Genetic traits		Sheep (14)	Goat (24)	Cattle (12)	Others^a (7)	Total (57)
Subtilase profiles^b
	subAB1			12		12
	subAB2-1		1		1^D,1^P	3
	subAB2-2	1				1
	subAB2-1/2-2	13	23		3^H, 2^D	41
Shiga toxin profiles
	stx1	11	14		3^H	28
	stx1/stx2	3	9		2^D, 1^P	15
	stx2		1	12	1^D	14
Sero-group
	O5	7				7
	O113		2	10	2^D,1^H	15
	O128		2			2
	Unknown	7	20	2	3^H,1^D	33
Phylo- group
	A		1	1	2^D,1^P	5
	B1	14	23	10	3^H,1^D	51
	E			1		1

^aOther sources are indicated as superscripts, H (Humans), D (Deer), P (Pony)

^bSubtilase AB2-3 was negative in all isolates

Shiga toxin genes and virulence determinants

The isolates from small ruminant harbored stx1, alone or in combination with stx2, but all cattle isolates only harbored the stx2 gene. Three human isolates possessed only the stx1, but most deer and pony strains harbored both stx1 and stx2 genes. As far as the additional virulence genes are concerned, tia was present in sheep, goats, deer, and pony isolates, but was not found in cattle or human strains. Interestingly, terD which encodes tellurite resistance was only found in deer and pony strains. Similarly, astA was detected in deer and pony strains and only in two goat isolates. Only one goat isolate belonging to O128 serogroup yielded the cdt amplicon. Among the plasmid-encoded virulence associated genes, ehly was present in most isolates (94.7%) regardless of the source, but the distribution of other virulence genes showed some correlations with the host. For instance, only cattle STEC carried espP and epeA, and none of the sheep and goat strains carried saa. None of the isolates carried toxB and katP, markers of the pO157 large virulence plasmid [26]. The adhesion genes iha and lpf ^O113 were present in most isolates belonging to different sources, while all strains tested were negative for efa1 (Table 3).

Phylogenetic groups and serogroups

Most strains belonged to phylogenetic group B1 (89.47%), while five strains from cattle, deer, pony and a goat were assigned to A phylogroup. Only one cattle isolate was designated as E phylogroup (Table 3). Among the tested serogroups, the most prevalent O-type was O113 (n=15), followed by O5 (n=7), and O128 (n=2). Interestingly, most cattle strain belonged to O113, while O5 was just detected in ovine isolates, and O128 and O113 were present in caprine strains (Table 3).

Table 3 Virulence gene combinations, subtilase variants, serogroups and phylo-groups of Shiga toxin-producing E. coli strains isolated from different reservoirs

Virulence gene profile

Sero-group

subAB

Source

Phylo-group

Total

stx1

stx2

ehly

tia

saa

espP

epeA

terD

astA

Lpf_O113

iha

1

2-1

2-2

+

O128

+

goat

B1

2^b

+

ND^a

+

sheep

B1

2

+

O5

+

sheep

B1

2

+

O5

+

sheep

B1

2

+

ND

+

sheep

B1

4

+

ND

+

goat

B1

6

+

ND

+

goat

B1

5

+

O5

+

sheep

B1

1

+

ND

+

human

B1

3

+

ND

+

goat

B1

1

+

O5

+

sheep

B1

2

+

ND

+

sheep

B1

1

+

ND

+

goat

B1

1

+

ND

+

goat

B1

7

+

O113

+

goat

A

1

+

O113

+

deer

A

1

+

O113

+

deer

A

1

+

O113

+

pony

A

1

+

ND

+

deer

B1

1

+

O113

+

goat

B1

1

+

ND

+

cattle

A

1

+

ND

+

cattle

E

1

+

O113

+

cattle

B1

10

^aNot-Defined

^bOne strain yielded an specific amplicon for cdt gene

Studies mostly conducted in the past decade unveiled that a subset of LEE- negative STEC can lead to sever conditions such as HUS in humans. The genetic lineages and evolution of such strains seem to be separated from the typical LEE-harboring strains. Accordingly, several specific virulence determinants including toxins, adhesins and invasion proteins have been discovered in the STEC strains lacking LEE pathogenicity island [5, 27]. Of the many definite or hypothetical virulence determinants present in these isolates, Subtilase-producing strains are believed to be one of the most important pathogenic lineages. With rare exceptions, subAB carriage seem to be almost exclusively associated with the STEC pathotype [28–30]. Subtilase not only acts as a potent toxin, but also occurs in different allelic variants in strains of different origins [12, 14, 15]. Recent findings suggested that different SubAB variants exhibit different binding capacity toward their target cells which may affect their cytotoxic behavior [29].

The subAB + E. coli has been frequently isolated from food-producing animals including cattle, sheep, goats, deer and large game animals in different countries; here, we reported its carriage in equine for the first time.

Overall, very few studies explored all subAB types because different allelic variants have not been elucidated until recently. Nevertheless, many studies confirmed that the carriage rate and allelic variants of subAB has been highly associated to the host species rather than the geographical origin of the strains. We similarly found that the subAB1 mainly occurs in cattle and subAB2 variants found in small ruminants, deer, horse and humans. We believe that such host specificity could be regarded as a primary tool for source tracking of disease epidemics due to LEE-negative STEC. In the present study, 24% of cattle, and 93 to 96% of sheep and goats carried variants of subAB1 and subAB2 (variants 1 and 2), respectively. Such carriage rate was strikingly similar to the other comprehensive research which found this gene in 25% of bovine and 91.9% of sheep and goats STEC in Spain [12]. In Brazil, 21 out of 95 STEC collection strains (22%) were positive in subA PCR which mainly targets the subAB1 [31]. Such surprising similarity in carriage of subAB may reflect the very old macro-evolutionary events in LEE- negative lineages which occurred in E. coli population within different hosts regardless of the geographical region. In other studies, the carriage rate of subAB2-1 was 86% in sheep and 72% in cases of human diarrhea [14]. In Spain, almost all caprine and ovine strains carried subAB2-2, but 61.4% and 64.3% carried subAB2-1 respectively [32]. In the present study, subAB2-1/2–2 variants occurred together in most isolates of sheep and goat strains (Table 2). Previously, one of the highest carriage rates has been reported in wild ruminants including ibex (100%) and Chamois (92%), but the rate was also high in Red deer (52.6%) and Roe deer (26.6%). In the mentioned study, one strain from a Roe deer harbored a new subAB2-3 in combination to subAB2-1, but 19 cattle isolates were negative for any subAB2 variants [15]. In our study subAB2 variants were present in all strains from captive wild ruminants but none included the new allelic type.

As mentioned, the pathogenicity of the LEE- negative strains can be reinforced by possession of various virulence determinants, some of which seem to be almost restricted to this subset of STEC [5, 27]. We found that along with subAB, strains harbor potential adhesins and invasion proteins such as iha, lpf ^O113, and tia at high rates, and include other markers such as saa, espP, epeA, and astA at lower frequencies. With these aforementioned markers, we also observed some host specificity. For example, the bovine strains mostly carried the combination of stx2/ehly/iha/ lpf^O113/epeA/espP/saa. This was not surprising as most of the cattle STEC belonged to O113 serogroup and many of such determinants are carried within pO113 mega plasmid [33]. Interestingly, four other STEC O113 from deer, goats and pony belonged to A phylogroup and exhibited different profiles as they lacked epeA and espP but carried stx1/stx2/ehly/tia/astA and saa (in 3 out of 4 strains). This suggests the presence of different plasmids in different O113 lineages in E. coli residing in different hosts, or the possible presence of chromosomal variants of some important genes such as ehly and saa in subAB2 carrying strains, which needs to be clarified in the future studies.

In conclusion, the present study showed for the first time the widespread presence of subAB variants in a large collection of STEC isolates in Iran. Our study clearly showed some host specific properties of subAB-harboring strains even within the same serogroup that makes typing of subAB variants a potential primary genetic tool that aids source tracking in outbreaks and epidemics due to LEE-negative STEC.

astA

EAEC heat-stable enterotoxin, cdt:cytolethal distending toxin, eae:E. coli attaching and effacing, efa:Enterohemorrhagic Escherichia coli factor for adherence, ehly:enterohemolysin, epeA:autotransporter protease, espP:Extracellular serine protease plasmid-encoded EspP, HC:hemorrhagic colitis, HUS:hemolytic uremic syndrome, Iha:bifunctional enterobactin receptor/adhesin protein, katP:catalase-peroxidase, LEE:locus of enterocyte effacement, lpf O113:long polar fimbria major subunit O113, OEP:outer membrane efflux protein locus, PCR:polymerase chain reaction, pO113:plasmid O113, saa:Shiga toxin-producing Escherichia coli autoagglutinating adhesion, SE-PAI:subtilase-encoding pathogenicity island, STEC:Shiga toxin-producing Escherichia coli, stx:Shiga toxin, subAB:subtilase, terD:tellurium resistance membrane protein TerD, tia:adhesion, toxB:putative cytotoxin B.

Ethics approval and consent to participate

All experimental protocols were approved by the committee for ethics in biomedical research in Veterinary Faculty of Ferdowsi University of Mashhad, Iran. Also, all methods were carried out in accordance with relevant guidelines and regulations presented by Iran National Committee for Ethics in Biomedical Research. We obtained informed consent from the owners of animals for sample collection by qualified persons.

Consent for publication

Not applicable.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Competing Interest

The authors declare that they have no competing interests.

Funding

There was no organizational financial support for this research.

Authors’ contributions

MA designed the study, analyzed the data and drafted the manuscript; MA, MJ and AR performed the main experiments; MA, MJ, TZS, and RG participated in the study design and data analysis; AK performed the complementary experiments and revised the draft. All authors read and approved the final manuscript.

Acknowledgments

The authors would like to express their gratitude to Zahra Izadkhah for excellent support in some laboratory procedures.

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No competing interests reported.

Distribution and Molecular Analysis of Subtilase Cytotoxin Gene (subAB) Variants in Shiga Toxin-Producing Escherichia Coli (STEC) Isolated From Different Sources in Iran

Status:

Version 1

Abstract

Background

Results

Conclusions

Background

Methods

Results

Discussion

Conclusions

Abbreviations

Declarations

References

Additional Declarations

Status:

Version 1