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Research article
Identification and functional characterization of NbMLP28, a novel MLP-like protein 28 enhancing Potato virus Y resistance in Nicotiana benthamiana
Yuanhua Wu
Shenyang Agricultural University
Corresponding Author
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Background: Major latex proteins (MLPs) belong to the MLP subfamily in Bet v 1 protein family and respond to both biotic and abiotic stresses, which play critical roles in plant disease resistance. As the type species of widely distributed and economically devastating Potyvirus, Potato virus Y (PVY) is one of the major constraints to important crop plants including tobacco ( Nicotiana benthamiana ) worldwide. Despite the great losses owing to PVY infection in tobacco, there is no previous study investigating the potential role of MLPs in developing resistance to viral infection.
Results: In this study, for the first time we have identified and functionally analyzed the MLP-like protein 28 from N. benthamiana , denoted as NbMLP28 and investigated its role in conferring resistance to N. benthamiana against PVY infection. NbMLP28 was localized to the plasmalemma and nucleus, with the highest level in the root. NbMLP28 gene was hypothesized to be triggered by PVY infection and was highly expressed in jasmonic acid (JA) signaling pathway. Further validation was achieved through silencing of NbMLP28 through virus-induced gene silencing (VIGS) that rendered N. benthamiana plants more vulnerable to PVY infection, contrary to overexpression that enhanced resistance.
Conclusions: Taken together, this is the first study describing the role of NbMLP28 in tobacco against PVY infection and provide a pivotal point towards obtaining pathogen-resistant tobacco varieties through constructing new candidate genes of MLP subfamily.
Keywords
Potato virus Y, resistance, N. benthamian, MLP-like proteins, NbMLP28, jasmonic acid, overexpression, VIGS, gene silencing
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CURRENT STATUS: Under Review
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Posted 15 Jan, 2020
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Review #2 received
Received 04 Feb, 2020
Reviewer #2 agreed
On 29 Jan, 2020
Reviewer #1 agreed
On 17 Jan, 2020
Review #1 received
Received 17 Jan, 2020
Reviewers invited
Invitations sent on 14 Jan, 2020
Editor assigned
On 13 Jan, 2020
Submission checks complete
On 12 Jan, 2020
Editor invited
On 12 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 03 Jan, 2020
Review #2 received
Received 27 Dec, 2019
Reviewer #2 agreed
On 19 Dec, 2019
Review #1 received
Received 10 Dec, 2019
Reviewer #1 agreed
On 02 Dec, 2019
Submission checks complete
On 28 Nov, 2019
Editor invited
On 28 Nov, 2019
Editor assigned
On 28 Nov, 2019
Reviewers invited
Invitations sent on 28 Nov, 2019
First submitted
On 16 Nov, 2019
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This preprint is under consideration at BMC Microbiology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Identification and functional characterization of NbMLP28, a novel MLP-like protein 28 enhancing Potato virus Y resistance in Nicotiana benthamiana
Yuanhua Wu
Shenyang Agricultural University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 2
Posted 15 Jan, 2020
No community comments so far
Review #2 received
Received 04 Feb, 2020
Reviewer #2 agreed
On 29 Jan, 2020
Reviewer #1 agreed
On 17 Jan, 2020
Review #1 received
Received 17 Jan, 2020
Reviewers invited
Invitations sent on 14 Jan, 2020
Editor assigned
On 13 Jan, 2020
Submission checks complete
On 12 Jan, 2020
Editor invited
On 12 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 03 Jan, 2020
Review #2 received
Received 27 Dec, 2019
Reviewer #2 agreed
On 19 Dec, 2019
Review #1 received
Received 10 Dec, 2019
Reviewer #1 agreed
On 02 Dec, 2019
Submission checks complete
On 28 Nov, 2019
Editor invited
On 28 Nov, 2019
Editor assigned
On 28 Nov, 2019
Reviewers invited
Invitations sent on 28 Nov, 2019
First submitted
On 16 Nov, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Major latex proteins (MLPs) belong to the MLP subfamily in Bet v 1 protein family and respond to both biotic and abiotic stresses, which play critical roles in plant disease resistance. As the type species of widely distributed and economically devastating Potyvirus, Potato virus Y (PVY) is one of the major constraints to important crop plants including tobacco ( Nicotiana benthamiana ) worldwide. Despite the great losses owing to PVY infection in tobacco, there is no previous study investigating the potential role of MLPs in developing resistance to viral infection.
Results: In this study, for the first time we have identified and functionally analyzed the MLP-like protein 28 from N. benthamiana , denoted as NbMLP28 and investigated its role in conferring resistance to N. benthamiana against PVY infection. NbMLP28 was localized to the plasmalemma and nucleus, with the highest level in the root. NbMLP28 gene was hypothesized to be triggered by PVY infection and was highly expressed in jasmonic acid (JA) signaling pathway. Further validation was achieved through silencing of NbMLP28 through virus-induced gene silencing (VIGS) that rendered N. benthamiana plants more vulnerable to PVY infection, contrary to overexpression that enhanced resistance.
Conclusions: Taken together, this is the first study describing the role of NbMLP28 in tobacco against PVY infection and provide a pivotal point towards obtaining pathogen-resistant tobacco varieties through constructing new candidate genes of MLP subfamily.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8