In the present study, PPAR-γ antagonist was used to induce preeclampsia model in mice to explore the intervention effect and mechanism of aspirin on preeclampsia model mice.
Pregnant mice were injected intraperitoneally with PPAR-γ antagonist (2 mg/kg/d) at 8.5-12.5 days of pregnancy to establish PE mouse models. Two doses of LDA (10 mg/kg/d and 20 mg/kg/d) were given to the PE mouse models for intervention.The blood pressure, 24-hour proteinuria, urine protein/creatinine ratio, interleukin (IL)1-β and endoglin level in peripheral blood, placental PPAR-γ mRNA expression in placenta, the placenta weight and pathology of placenta and kidneys of mice from different groups were detected.
LDA effectively decreased the blood pressure increase caused by PPAR-γ antagonist in mice, and the increase degree of urinary protein and urinary protein creatinine ratio was notably relieved.LDA also inhibited the overexpression of Endoglin and IL-β induced by PPAR-γ antagonist.In addition, LDA evidently increased the placental weight of preeclampsia mice.The placenta and kidney injury were obviously less serious.LDA alleviated the expression inhibition of PPAR-γ mRNA.The remission effect of 20mgLDA was significantly better than that of 10mg.
(1) LDA has a preventive effect in PE mice induced by PPAR-γ antagonist. (2) The preventive effect of LDA is dose-dependent in PE mouse models induced by PPAR-γ antagonist.