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Carbon Nanoparticles Enhance Potassium Uptake via Upregulating Potassium Channel Expression and Imitating Biological Ion Channels in BY-2 Cells
Jianping Xie
Zhengzhou Tobacco Research Institute
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8946-5597
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Journal of Nanobiotechnology.
Background: Carbon nanoparticles (CNPs) have been reported to boost plant growth, while the mechanism that CNPs enhanced potassium uptake for plant growth have not been reported so far. Results: In this study, the function that CNPs promoted potassium uptake in BY-2 cells was established and the potassium accumulated in cells had a significant correlation with the fresh biomass of BY-2 cells. The K+ accumulation in cells increased with the increasing concentration of CNPs. The K+ influx reached high level after treatment with CNPs and was significantly higher than that of the control group and the negative group treated with K+ channels blocker, tetraethylammonium chloride (TEA+). The K+ accumulation was not reduced in the presence of CNPs inhibitors. In the presence of potassium channel blocker TEA+ or CNPs inhibitors, the NKT1 gene expression was changed compared with the control group. The CNPs were found to preferentially transport K+ than other cations determined by rectification of ion current assay (RIC) in a conical nanocapillary. Conclusions: These results indicated that CNPs upregulated potassium gene expression to enhance K+ accumulation in BY-2 cells. Moreover, it was speculated that the CNPs simulated protein of ion channels via bulk of carboxyl for K+ permeating. These findings will provide support for improving plant growth by carbon nanoparticles.
Keywords
carbon nanoparticles, potassium uptake, gene expression, ion channels
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CURRENT STATUS: Published
Version 2
Posted 16 Jan, 2020
Published
On 28 Jan, 2020
No community comments so far
Editorial decision: Accept
On 15 Jan, 2020
Editor assigned
On 14 Jan, 2020
Submission checks complete
On 13 Jan, 2020
Editor invited
On 13 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 30 Dec, 2019
Review #2 received
Received 27 Dec, 2019
Reviewer #3 agreed
On 15 Dec, 2019
Review #1 received
Received 14 Dec, 2019
Reviewer #2 agreed
On 13 Dec, 2019
Reviewer #1 agreed
On 03 Dec, 2019
Editor assigned
On 02 Dec, 2019
Reviewers invited
Invitations sent on 02 Dec, 2019
Submission checks complete
On 01 Dec, 2019
Editor invited
On 01 Dec, 2019
First submitted
On 28 Nov, 2019
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Subject Areas
Nanoscience
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This preprint is under consideration at Journal of Nanobiotechnology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Carbon Nanoparticles Enhance Potassium Uptake via Upregulating Potassium Channel Expression and Imitating Biological Ion Channels in BY-2 Cells
Jianping Xie
Zhengzhou Tobacco Research Institute
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8946-5597
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 16 Jan, 2020
Published
On 28 Jan, 2020
No community comments so far
Editorial decision: Accept
On 15 Jan, 2020
Editor assigned
On 14 Jan, 2020
Submission checks complete
On 13 Jan, 2020
Editor invited
On 13 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 30 Dec, 2019
Review #2 received
Received 27 Dec, 2019
Reviewer #3 agreed
On 15 Dec, 2019
Review #1 received
Received 14 Dec, 2019
Reviewer #2 agreed
On 13 Dec, 2019
Reviewer #1 agreed
On 03 Dec, 2019
Editor assigned
On 02 Dec, 2019
Reviewers invited
Invitations sent on 02 Dec, 2019
Submission checks complete
On 01 Dec, 2019
Editor invited
On 01 Dec, 2019
First submitted
On 28 Nov, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Nanoscience
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Journal of Nanobiotechnology.
Background: Carbon nanoparticles (CNPs) have been reported to boost plant growth, while the mechanism that CNPs enhanced potassium uptake for plant growth have not been reported so far. Results: In this study, the function that CNPs promoted potassium uptake in BY-2 cells was established and the potassium accumulated in cells had a significant correlation with the fresh biomass of BY-2 cells. The K+ accumulation in cells increased with the increasing concentration of CNPs. The K+ influx reached high level after treatment with CNPs and was significantly higher than that of the control group and the negative group treated with K+ channels blocker, tetraethylammonium chloride (TEA+). The K+ accumulation was not reduced in the presence of CNPs inhibitors. In the presence of potassium channel blocker TEA+ or CNPs inhibitors, the NKT1 gene expression was changed compared with the control group. The CNPs were found to preferentially transport K+ than other cations determined by rectification of ion current assay (RIC) in a conical nanocapillary. Conclusions: These results indicated that CNPs upregulated potassium gene expression to enhance K+ accumulation in BY-2 cells. Moreover, it was speculated that the CNPs simulated protein of ion channels via bulk of carboxyl for K+ permeating. These findings will provide support for improving plant growth by carbon nanoparticles.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8