A severe flooding hit southern China along the Yangtze River in summer 2020. The floods were induced by heavy rains, and the associated dynamic and thermodynamic conditions are investigated using daily gridded rainfall data of China and NCEP-NCAR reanalysis. It is found that the summer rainfall over the Yangtze River Basin (YRB) experienced pronounced subseasonal variation in 2020, dominated by a quasi-biweekly oscillation (QBWO) mode. The southwestward-moving anomalous QBWO circulation was essentially the fluctuation of cold air mass related to the tropospheric polar vortex or trough-ridge activities over the mid-high latitude Eurasian in boreal summer. The large-scale southwestward-transport of cold air mass from mid-high latitudes and the northeastward-transport of warm and moist air by the strong anomalous anticyclone over the western North Pacific provided important circulation support for the heavy rainfall in the YRB. The quasi-biweekly anomalies of potential and divergent component of vertically integrated water vapor flux played a major role in maintaining the moisture during summer 2020. The diagnosis of moisture budget shows that the enhanced moisture associated with the quasi-biweekly fluctuation rainfall was primarily attributed to the moisture convergence. The convergence of QBWO specific humidity by the background mean flow and convergence of mean specific humidity by QBWO flow played dominant roles in contributing to the positive moisture tendency. In combination with an adiabatic ascent induced by the warm temperature advection, the boundary layer moisture convergence strengthens the upward transport of moisture from lower troposphere. The vertical moisture transport associated with boundary layer convergence was of critical importance in causing low-level tropospheric moistening, whereas the horizontal advection of moisture showed a negative effect during the anomalous quasi-biweekly summer rainfall in 2020.