1 Macosko, E. Z. et al. Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell161, 1202-1214, doi:10.1016/j.cell.2015.05.002 (2015).
2 Klein, A. M. et al. Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells. Cell161, 1187-1201, doi:10.1016/j.cell.2015.04.044 (2015).
3 Stoeckius, M. et al. Simultaneous epitope and transcriptome measurement in single cells. Nat Methods14, 865-868, doi:10.1038/nmeth.4380 (2017).
4 Choi, Y. J., Park, H. J., Park, H. J., Jung, K. C. & Lee, J. I. CD4(hi)CD8(low) Double-Positive T Cells Are Associated with Graft Rejection in a Nonhuman Primate Model of Islet Transplantation. J Immunol Res2018, 3861079, doi:10.1155/2018/3861079 (2018).
5 Zuckermann, F. A. Extrathymic CD4/CD8 double positive T cells. Veterinary immunology and immunopathology72, 55-66 (1999).
6 Bohner, P. et al. Double Positive CD4(+)CD8(+) T Cells Are Enriched in Urological Cancers and Favor T Helper-2 Polarization. Front Immunol10, 622, doi:10.3389/fimmu.2019.00622 (2019).
7 Desfrancois, J. et al. Double positive CD4CD8 alphabeta T cells: a new tumor-reactive population in human melanomas. PloS one5, e8437, doi:10.1371/journal.pone.0008437 (2010).
8 Parel, Y. & Chizzolini, C. CD4+ CD8+ double positive (DP) T cells in health and disease. Autoimmun Rev3, 215-220, doi:10.1016/j.autrev.2003.09.001 (2004).
9 Nascimbeni, M., Pol, S. & Saunier, B. Distinct CD4+ CD8+ double-positive T cells in the blood and liver of patients during chronic hepatitis B and C. PloS one6, e20145, doi:10.1371/journal.pone.0020145 (2011).
10 Nascimbeni, M., Shin, E. C., Chiriboga, L., Kleiner, D. E. & Rehermann, B. Peripheral CD4(+)CD8(+) T cells are differentiated effector memory cells with antiviral functions. Blood104, 478-486, doi:10.1182/blood-2003-12-4395 (2004).
11 Akari, H., Terao, K., Murayama, Y., Nam, K. H. & Yoshikawa, Y. Peripheral blood CD4+CD8+ lymphocytes in cynomolgus monkeys are of resting memory T lineage. International immunology9, 591-597 (1997).
12 Van Kaer, L., Rabacal, W. A., Scott Algood, H. M., Parekh, V. V. & Olivares-Villagomez, D. In vitro induction of regulatory CD4+CD8alpha+ T cells by TGF-beta, IL-7 and IFN-gamma. PloS one8, e67821, doi:10.1371/journal.pone.0067821 (2013).
13 Quandt, D., Rothe, K., Scholz, R., Baerwald, C. W. & Wagner, U. Peripheral CD4CD8 double positive T cells with a distinct helper cytokine profile are increased in rheumatoid arthritis. PloS one9, e93293, doi:10.1371/journal.pone.0093293 (2014).
14 Kerstein, A., Muller, A., Pitann, S., Riemekasten, G. & Lamprecht, P. Circulating CD4+CD8+ double-positive T-cells display features of innate and adaptive immune function in granulomatosis with polyangiitis. Clin Exp Rheumatol36 Suppl 111, 93-98 (2018).
15 Sheng, H. M. et al. Distinct PLZF(+)CD8 alpha alpha(+) Unconventional T Cells Enriched in Liver Use a Cytotoxic Mechanism to Limit Autoimmunity. Journal of immunology203, 2150-2162, doi:10.4049/jimmunol.1900832 (2019).
16 Jacomet, F. et al. Evidence for eomesodermin-expressing innate-like CD8(+) KIR/NKG2A(+) T cells in human adults and cord blood samples. Eur J Immunol45, 1926-1933, doi:10.1002/eji.201545539 (2015).
17 Hori, S., Nomura, T. & Sakaguchi, S. Control of regulatory T cell development by the transcription factor Foxp3. Science299, 1057-1061, doi:10.1126/science.1079490 (2003).
18 Sullivan, Y. B., Landay, A. L., Zack, J. A., Kitchen, S. G. & Al-Harthi, L. Upregulation of CD4 on CD8+ T cells: CD4dimCD8bright T cells constitute an activated phenotype of CD8+ T cells. Immunology103, 270-280 (2001).
19 Park, J. E. et al. A cell atlas of human thymic development defines T cell repertoire formation. Science367, doi:10.1126/science.aay3224 (2020).
20 Kakugawa, K. et al. Essential Roles of SATB1 in Specifying T Lymphocyte Subsets. Cell Rep19, 1176-1188, doi:10.1016/j.celrep.2017.04.038 (2017).
21 Hollbacher, B. et al. Transcriptomic Profiling of Human Effector and Regulatory T Cell Subsets Identifies Predictive Population Signatures. Immunohorizons4, 585-596, doi:10.4049/immunohorizons.2000037 (2020).
22 Hashimoto, K. et al. Single-cell transcriptomics reveals expansion of cytotoxic CD4 T cells in supercentenarians. Proceedings of the National Academy of Sciences of the United States of America116, 24242-24251, doi:10.1073/pnas.1907883116 (2019).
23 Patil, V. S. et al. Precursors of human CD4(+) cytotoxic T lymphocytes identified by single-cell transcriptome analysis. Sci Immunol3, doi:10.1126/sciimmunol.aan8664 (2018).
24 Bonnal, R. J. P. et al. Clonally expanded EOMES(+) Tr1-like cells in primary and metastatic tumors are associated with disease progression. Nat Immunol22, 735-745, doi:10.1038/s41590-021-00930-4 (2021).
25 Jeong, A. R., Nakamura, S. & Mitsunaga, F. Gene expression profile of Th1 and Th2 cytokines and their receptors in human and nonhuman primates. Journal of medical primatology37, 290-296, doi:10.1111/j.1600-0684.2008.00289.x (2008).
26 Singh, S. P. et al. PLZF Regulates CCR6 and Is Critical for the Acquisition and Maintenance of the Th17 Phenotype in Human Cells. Journal of immunology194, 4350-4361, doi:10.4049/jimmunol.1401093 (2015).
27 Schmitt, N., Bentebibel, S. E. & Ueno, H. Phenotype and functions of memory Tfh cells in human blood. Trends Immunol35, 436-442, doi:10.1016/j.it.2014.06.002 (2014).
28 Hidalgo, L. G. et al. The transcriptome of human cytotoxic T cells: measuring the burden of CTL-associated transcripts in human kidney transplants. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons8, 637-646, doi:10.1111/j.1600-6143.2007.02129.x (2008).
29 Correia, M. P. et al. Distinct human circulating NKp30(+)FcepsilonRIgamma(+)CD8(+) T cell population exhibiting high natural killer-like antitumor potential. Proceedings of the National Academy of Sciences of the United States of America115, E5980-E5989, doi:10.1073/pnas.1720564115 (2018).
30 Huang, Y. et al. Mucosal memory CD8(+) T cells are selected in the periphery by an MHC class I molecule. Nat Immunol12, 1086-1095, doi:10.1038/ni.2106 (2011).
31 Tian, Y., Sette, A. & Weiskopf, D. Cytotoxic CD4 T Cells: Differentiation, Function, and Application to Dengue Virus Infection. Front Immunol7, 531, doi:10.3389/fimmu.2016.00531 (2016).
32 Yamamoto, J. et al. Differential expression of the chemokine receptors by the Th1-and Th2-type effector populations within circulating CD4(+) T cells. J Leukocyte Biol68, 568-574 (2000).
33 Annunziato, F. et al. Phenotypic and functional features of human Th17 cells. The Journal of experimental medicine204, 1849-1861, doi:10.1084/jem.20070663 (2007).
34 He, J. et al. Circulating precursor CCR7(lo)PD-1(hi) CXCR5(+) CD4(+) T cells indicate Tfh cell activity and promote antibody responses upon antigen reexposure. Immunity39, 770-781, doi:10.1016/j.immuni.2013.09.007 (2013).
35 Chavele, K. M., Merry, E. & Ehrenstein, M. R. Cutting edge: circulating plasmablasts induce the differentiation of human T follicular helper cells via IL-6 production. Journal of immunology194, 2482-2485, doi:10.4049/jimmunol.1401190 (2015).
36 Gruarin, P. et al. Eomesodermin controls a unique differentiation program in human IL-10 and IFN-gamma coproducing regulatory T cells. Eur J Immunol49, 96-111, doi:10.1002/eji.201847722 (2019).
37 Overgaard, N. H., Jung, J. W., Steptoe, R. J. & Wells, J. W. CD4+/CD8+ double-positive T cells: more than just a developmental stage? J Leukoc Biol97, 31-38, doi:10.1189/jlb.1RU0814-382 (2015).
38 Das, G. et al. An important regulatory role for CD4+CD8 alpha alpha T cells in the intestinal epithelial layer in the prevention of inflammatory bowel disease. Proceedings of the National Academy of Sciences of the United States of America100, 5324-5329, doi:10.1073/pnas.0831037100 (2003).
39 Walker, L. J. et al. CD8alphaalpha Expression Marks Terminally Differentiated Human CD8+ T Cells Expanded in Chronic Viral Infection. Front Immunol4, 223, doi:10.3389/fimmu.2013.00223 (2013).
40 Parrot, T. et al. Transcriptomic features of tumour-infiltrating CD4(low)CD8(high) double positive alpha beta T cells in melanoma. Scientific reports10, doi:ARTN 5900. 10.1038/s41598-020-62664-x (2020).
41 Fergusson, J. R., Fleming, V. M. & Klenerman, P. CD161-expressing human T cells. Front Immunol2, 36, doi:10.3389/fimmu.2011.00036 (2011).
42 Alonzo, E. S. & Sant'Angelo, D. B. Development of PLZF-expressing innate T cells. Curr Opin Immunol23, 220-227, doi:10.1016/j.coi.2010.12.016 (2011).
43 Libreros, S. & Iragavarapu-Charyulu, V. YKL-40/CHI3L1 drives inflammation on the road of tumor progression. J Leukoc Biol98, 931-936, doi:10.1189/jlb.3VMR0415-142R (2015).
44 Ahmed, R. et al. A Public BCR Present in a Unique Dual-Receptor-Expressing Lymphocyte from Type 1 Diabetes Patients Encodes a Potent T Cell Autoantigen. Cell177, 1583-1599 e1516, doi:10.1016/j.cell.2019.05.007 (2019).