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miR-147b-modulated expression of vestigial regulates wing development in the bird cherry-oat aphid Rhopalosiphum padimiR-147b-modulated expression of vestigial regulates wing development in the bird cherry-oat aphid Rhopalosiphum padi
Xiwu Gao
China Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3854-2449
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomics.
Background: Most aphids exhibit wing polyphenism in which wingless and winged morphs produce depending on the population density and host plant quality. Although the influence of environmental factors on wing polyphenism of aphids have been extensively investigated, molecular mechanisms underlining morph differentiation (i.e. wing development /degeneration), one downstream aspect of the wing polyphenism , has been poorly understood. Results: We examined the expression levels of the twenty genes involved in wing development network, and only vestigial (vg ) showed significantly different expression levels in both whole-body and wall-body of third instar nymphs, with 5.4- and 16.14- fold higher expression in winged lines compared to wingless lines, respectively in Rhopalosiphum padi . vg expression was higher in winged lines compared to wingless lines in third, fourth instar nymphs and adults. Larger difference expression was observed in third (21.38-fold) and fourth (20.91-fold) instar nymphs relative to adults (3.12-fold). Suppression of vg using RNAi repressed the wing development of third winged morphs. Furthermore, dual luciferase reporter assay revealed that the miR-147 can target the vg mRNA. Modulation of miR-147b levels by microinjection of its agomir (mimic) decreased vg expression levels and repressed wing development. Conclusions : Our findings suggest that vg is essential for wing development in R. padi and that miR-147b modulates its expression. .
Keywords
Wing development, Vestigial, MicroRNA, Rhopalosiphum padi
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Published
On 22 Jan, 2020
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On 08 Jan, 2020
Editorial decision: Accept
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No comments provided
Editorial decision: Minor revision
On 23 Dec, 2019
Editor assigned
On 02 Dec, 2019
Submission checks complete
On 01 Dec, 2019
Editor invited
On 01 Dec, 2019
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Editorial decision: Minor revision
On 17 Nov, 2019
Review #2 received
Received 17 Oct, 2019
Review #1 received
Received 11 Oct, 2019
Reviewers invited
Invitations sent on 07 Oct, 2019
Reviewer #1 agreed
On 07 Oct, 2019
Reviewer #2 agreed
On 07 Oct, 2019
Editor assigned
On 16 Sep, 2019
Submission checks complete
On 15 Sep, 2019
Editor invited
On 15 Sep, 2019
No comments provided
Editorial decision: Major revision
On 08 Jul, 2019
Review #2 received
Received 06 Jul, 2019
Reviewer #2 agreed
On 05 Jul, 2019
Review #1 received
Received 17 Jun, 2019
Reviewer #1 agreed
On 11 Jun, 2019
Reviewers invited
Invitations sent on 19 May, 2019
Submission checks complete
On 15 May, 2019
Editor invited
On 03 May, 2019
Editor assigned
On 03 May, 2019
First submitted
On 27 Apr, 2019
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Subject Areas
Epigenetics & Genomics
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This preprint is under consideration at BMC Genomics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
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Research article
miR-147b-modulated expression of vestigial regulates wing development in the bird cherry-oat aphid Rhopalosiphum padimiR-147b-modulated expression of vestigial regulates wing development in the bird cherry-oat aphid Rhopalosiphum padi
Xiwu Gao
China Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3854-2449
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 4
Posted 10 Jan, 2020
Published
On 22 Jan, 2020
No community comments so far
Submission checks complete
On 08 Jan, 2020
Editorial decision: Accept
On 08 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 23 Dec, 2019
Editor assigned
On 02 Dec, 2019
Submission checks complete
On 01 Dec, 2019
Editor invited
On 01 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 17 Nov, 2019
Review #2 received
Received 17 Oct, 2019
Review #1 received
Received 11 Oct, 2019
Reviewers invited
Invitations sent on 07 Oct, 2019
Reviewer #1 agreed
On 07 Oct, 2019
Reviewer #2 agreed
On 07 Oct, 2019
Editor assigned
On 16 Sep, 2019
Submission checks complete
On 15 Sep, 2019
Editor invited
On 15 Sep, 2019
No comments provided
Editorial decision: Major revision
On 08 Jul, 2019
Review #2 received
Received 06 Jul, 2019
Reviewer #2 agreed
On 05 Jul, 2019
Review #1 received
Received 17 Jun, 2019
Reviewer #1 agreed
On 11 Jun, 2019
Reviewers invited
Invitations sent on 19 May, 2019
Submission checks complete
On 15 May, 2019
Editor invited
On 03 May, 2019
Editor assigned
On 03 May, 2019
First submitted
On 27 Apr, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomics.
Background: Most aphids exhibit wing polyphenism in which wingless and winged morphs produce depending on the population density and host plant quality. Although the influence of environmental factors on wing polyphenism of aphids have been extensively investigated, molecular mechanisms underlining morph differentiation (i.e. wing development /degeneration), one downstream aspect of the wing polyphenism , has been poorly understood. Results: We examined the expression levels of the twenty genes involved in wing development network, and only vestigial (vg ) showed significantly different expression levels in both whole-body and wall-body of third instar nymphs, with 5.4- and 16.14- fold higher expression in winged lines compared to wingless lines, respectively in Rhopalosiphum padi . vg expression was higher in winged lines compared to wingless lines in third, fourth instar nymphs and adults. Larger difference expression was observed in third (21.38-fold) and fourth (20.91-fold) instar nymphs relative to adults (3.12-fold). Suppression of vg using RNAi repressed the wing development of third winged morphs. Furthermore, dual luciferase reporter assay revealed that the miR-147 can target the vg mRNA. Modulation of miR-147b levels by microinjection of its agomir (mimic) decreased vg expression levels and repressed wing development. Conclusions : Our findings suggest that vg is essential for wing development in R. padi and that miR-147b modulates its expression. .
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7