Background:Cardiomyopathy is a disease with a very low cure rate and a very complex pathogenesis.Although genes are known to play an important role in
various types of cardiomyopathy, the specific mechanism has not been well studied.
Methods:We screened the GSE29819 dataset from the Gene Expression Omnibus database (GEO) for long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) with significant differences using R software. microRNAs (miRNAs) regulated by the lncRNAs were retrieved from the miRcode database. The downstream target genes of the miRNAs were predicted by miRDB, miRTarBase, and TargetScan, and genes consistent with the original dataset were selected to construct a lncRNA–miRNA–mRNA network. Finally, the biological effects of these genes were studied by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis.
Results: 223 differentially expressed genes and 13 differentially expressed lncRNAs has been screened in the cardiomyopathy tissues.After database screening and matching, the final lncRNA–miRNA–mRNA network included three lncRNAs, eight miRNAs, and nine mRNAs. These nine genes are mainly involved in the prolactin signaling pathway, EGFR tyrosine kinase inhibitor resistance, toxoplasmosis, the chemokine signaling pathway, and the PI3K–Akt signaling pathway. In the PI3K–Akt signaling pathway, the expression levels of these genes are significantly upregulated compared with the control group, and the main genes involved in regulation are JAK2/DDIT4/FOXO3. JAK2 and FOXO3 play an important regulatory role in the PI3K–Akt signaling pathway.
Conclusion:The AC017002–miRNA-590-5p–FOXO3 network may be involved in the pathogenesis of cardiomyopathy, which will enable the determination of new markers to predict cardiomyopathy, and thus reduce the risk of developing the disease.