Heart failure is caused by various factors, making its underlying pathogenic mechanisms difficult to identify, and tends to worsen over time. Early diagnosis of cardiovascular disease is the key for treatment to promote healthy life. To detect the structural and functional molecular changes associated with cardiovascular disease, we focused on glycans, which reflect the type and state of cells. We investigated glycan localization in the cardiac tissue of normal mice and their alterations during aging using an evanescent-filed lectin microarray, a technique based on lectin-glycan interaction, and lectin staining. The glycan profiles in the left ventricle showed differences between the luminal side (medial) and the wall side (lateral) region. The former area was characterized by the presence of sialic acid residues.Moreover, age-related changes in glycan profiles were observed earlier in the medial region. The difference in the age-related decrease of a-galactose stained with griffonia simplicifolia lectin-IB4 in different region of the leftventricle suggested spatiotemporal changes in microvessels. The glycan profile, which retains diverse glycan structures, is supported by many cell populations and maintains cardiac function. Glycan localization and changes are expected to be developed as a marker of the signs and symptoms of heart failure in the future.