Background: Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome editing tools, such as CRISPR/Cas9, can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease.
Results: In this study, we constructed a CRISPR/Cas9 vector construct targeting the canine DJ-1 gene. Genome-edited canine fibroblasts were established by transfection of the vector following antibiotic selection. We performed canine SCNT using genome-edited fibroblasts and successfully produced two genome-edited dogs. Both genome-edited dogs had indel mutations at the target locus, and expression of the DJ-1 gene was downregulated or completely repressed.
Conclusion: In conclusion, SCNT successfully produced genome-edited dogs using the CRISPR/Cas9 system for the first time.