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Genome Wide Identification and Characterization of Light-Harvesting Chloro a/b Binding Genes Reveals their Potential Role in Enhancing Drought Tolerance in Gossypium hirsutum
Fang Liu
Chinese Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6213-9572
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Cotton is an important commercial crop for its valuable source of natural fiber. Its production has undergone a sharp failure because of abiotic stress influences, of significance is drought. Moreover, plants have evolved self-defense mechanisms against the effects of several ways of abiotic factors like drought, salt, cold among others. The evolution of stress responsive transcription factors such as the trihelix, a nodule-inception-like protein (NLP), the late embryogenesis abundant (LEA) proteins among others have shown positive response in improving resistance to several forms of abiotic stress features.
Results
Genome wide identification and characterization of the effects of Light-Harvesting Chloro a/b binding (LHC) genes was carried out in cotton under drought stress conditions. A hundred and nine proteins encoded by the LHC genes were found in the cotton genome, with 55, 27, and 27 genes found to be distributed in Gossypium hirsutum, G. arboreum, and G. raimondii, respectively. The proteins encoded by the genes were unevenly distributed in various chromosomes. The Ka/Ks values were less than one, and an indication of negative selection of the gene family. differential expression arrangement of genes was showed with the majority of the genes being highly upregulated in the root tissues in relative to leave and stem tissues. Moreover, more genes were induced in M85 a relative drought tolerant germplasm.
Conclusion:
The results provide proof of the possible role of the LHC genes in improving drought stress tolerance, and can be explored by cotton breeders in releasing a more drought tolerant cotton germplasms.
Keywords
Cotton, G. hirsutum, LHC genes, Gene expression, Drought tolerance
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This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.doc
List of Primer details of RT-qPCR for LHC genes; Primers was designed by NCBI primer blast
- TableS2.xlsx
List of Light Harvesting Chloro a-b-binding Genes in G. hirsutum, G. arboreum, and G. raimondii respectively
- TableS3.xlsx
Ka, Ks, Ka/Ks values of LHC genes.
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CURRENT STATUS: Under Revision
Version 1
Posted 30 Oct, 2020
No community comments so far
Editorial decision: Major revision
On 19 Feb, 2021
Review #4 received
Received 25 Jan, 2021
Review #2 received
Received 15 Jan, 2021
Review #1 received
Received 15 Jan, 2021
Review #3 received
Received 10 Jan, 2021
Reviewer #4 agreed
On 08 Jan, 2021
Reviewer #3 agreed
On 29 Dec, 2020
Reviewer #2 agreed
On 28 Dec, 2020
Reviewer #1 agreed
On 25 Nov, 2020
Reviewers invited
Invitations sent on 24 Nov, 2020
Editor assigned
On 26 Oct, 2020
Submission checks complete
On 25 Oct, 2020
Editor invited
On 25 Oct, 2020
First submitted
On 23 Oct, 2020
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Subject Areas
Plant Molecular Biology and Genetics
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This preprint is under consideration at Journal of Cotton Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Genome Wide Identification and Characterization of Light-Harvesting Chloro a/b Binding Genes Reveals their Potential Role in Enhancing Drought Tolerance in Gossypium hirsutum
Fang Liu
Chinese Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6213-9572
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 30 Oct, 2020
No community comments so far
Editorial decision: Major revision
On 19 Feb, 2021
Review #4 received
Received 25 Jan, 2021
Review #2 received
Received 15 Jan, 2021
Review #1 received
Received 15 Jan, 2021
Review #3 received
Received 10 Jan, 2021
Reviewer #4 agreed
On 08 Jan, 2021
Reviewer #3 agreed
On 29 Dec, 2020
Reviewer #2 agreed
On 28 Dec, 2020
Reviewer #1 agreed
On 25 Nov, 2020
Reviewers invited
Invitations sent on 24 Nov, 2020
Editor assigned
On 26 Oct, 2020
Submission checks complete
On 25 Oct, 2020
Editor invited
On 25 Oct, 2020
First submitted
On 23 Oct, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Molecular Biology and Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Cotton is an important commercial crop for its valuable source of natural fiber. Its production has undergone a sharp failure because of abiotic stress influences, of significance is drought. Moreover, plants have evolved self-defense mechanisms against the effects of several ways of abiotic factors like drought, salt, cold among others. The evolution of stress responsive transcription factors such as the trihelix, a nodule-inception-like protein (NLP), the late embryogenesis abundant (LEA) proteins among others have shown positive response in improving resistance to several forms of abiotic stress features.
Results
Genome wide identification and characterization of the effects of Light-Harvesting Chloro a/b binding (LHC) genes was carried out in cotton under drought stress conditions. A hundred and nine proteins encoded by the LHC genes were found in the cotton genome, with 55, 27, and 27 genes found to be distributed in Gossypium hirsutum, G. arboreum, and G. raimondii, respectively. The proteins encoded by the genes were unevenly distributed in various chromosomes. The Ka/Ks values were less than one, and an indication of negative selection of the gene family. differential expression arrangement of genes was showed with the majority of the genes being highly upregulated in the root tissues in relative to leave and stem tissues. Moreover, more genes were induced in M85 a relative drought tolerant germplasm.
Conclusion:
The results provide proof of the possible role of the LHC genes in improving drought stress tolerance, and can be explored by cotton breeders in releasing a more drought tolerant cotton germplasms.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8