Genome Wide Identification and Characterization of Light-Harvesting Chloro a/b Binding Genes Reveals their Potential Role in Enhancing Drought Tolerance in Gossypium hirsutum
Background
Cotton is an important commercial crop for its valuable source of natural fiber. Its production has undergone a sharp failure because of abiotic stress influences, of significance is drought. Moreover, plants have evolved self-defense mechanisms against the effects of several ways of abiotic factors like drought, salt, cold among others. The evolution of stress responsive transcription factors such as the trihelix, a nodule-inception-like protein (NLP), the late embryogenesis abundant (LEA) proteins among others have shown positive response in improving resistance to several forms of abiotic stress features.
Results
Genome wide identification and characterization of the effects of Light-Harvesting Chloro a/b binding (LHC) genes was carried out in cotton under drought stress conditions. A hundred and nine proteins encoded by the LHC genes were found in the cotton genome, with 55, 27, and 27 genes found to be distributed in Gossypium hirsutum, G. arboreum, and G. raimondii, respectively. The proteins encoded by the genes were unevenly distributed in various chromosomes. The Ka/Ks values were less than one, and an indication of negative selection of the gene family. differential expression arrangement of genes was showed with the majority of the genes being highly upregulated in the root tissues in relative to leave and stem tissues. Moreover, more genes were induced in M85 a relative drought tolerant germplasm.
Conclusion:
The results provide proof of the possible role of the LHC genes in improving drought stress tolerance, and can be explored by cotton breeders in releasing a more drought tolerant cotton germplasms.
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This is a list of supplementary files associated with this preprint. Click to download.
List of Primer details of RT-qPCR for LHC genes; Primers was designed by NCBI primer blast
List of Light Harvesting Chloro a-b-binding Genes in G. hirsutum, G. arboreum, and G. raimondii respectively
Ka, Ks, Ka/Ks values of LHC genes.
Posted 30 Oct, 2020
On 19 Feb, 2021
Received 25 Jan, 2021
Received 15 Jan, 2021
Received 15 Jan, 2021
Received 10 Jan, 2021
On 08 Jan, 2021
On 29 Dec, 2020
On 28 Dec, 2020
On 25 Nov, 2020
Invitations sent on 24 Nov, 2020
On 26 Oct, 2020
On 25 Oct, 2020
On 25 Oct, 2020
On 23 Oct, 2020
Genome Wide Identification and Characterization of Light-Harvesting Chloro a/b Binding Genes Reveals their Potential Role in Enhancing Drought Tolerance in Gossypium hirsutum
Posted 30 Oct, 2020
On 19 Feb, 2021
Received 25 Jan, 2021
Received 15 Jan, 2021
Received 15 Jan, 2021
Received 10 Jan, 2021
On 08 Jan, 2021
On 29 Dec, 2020
On 28 Dec, 2020
On 25 Nov, 2020
Invitations sent on 24 Nov, 2020
On 26 Oct, 2020
On 25 Oct, 2020
On 25 Oct, 2020
On 23 Oct, 2020
Background
Cotton is an important commercial crop for its valuable source of natural fiber. Its production has undergone a sharp failure because of abiotic stress influences, of significance is drought. Moreover, plants have evolved self-defense mechanisms against the effects of several ways of abiotic factors like drought, salt, cold among others. The evolution of stress responsive transcription factors such as the trihelix, a nodule-inception-like protein (NLP), the late embryogenesis abundant (LEA) proteins among others have shown positive response in improving resistance to several forms of abiotic stress features.
Results
Genome wide identification and characterization of the effects of Light-Harvesting Chloro a/b binding (LHC) genes was carried out in cotton under drought stress conditions. A hundred and nine proteins encoded by the LHC genes were found in the cotton genome, with 55, 27, and 27 genes found to be distributed in Gossypium hirsutum, G. arboreum, and G. raimondii, respectively. The proteins encoded by the genes were unevenly distributed in various chromosomes. The Ka/Ks values were less than one, and an indication of negative selection of the gene family. differential expression arrangement of genes was showed with the majority of the genes being highly upregulated in the root tissues in relative to leave and stem tissues. Moreover, more genes were induced in M85 a relative drought tolerant germplasm.
Conclusion:
The results provide proof of the possible role of the LHC genes in improving drought stress tolerance, and can be explored by cotton breeders in releasing a more drought tolerant cotton germplasms.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8