Antioxidant enzymes protect the human body against the harmful effects of oxidative stress. The activity of antioxidant enzymes changes with age, and depends on dietary nutrients such as fats and vitamins, which can have a significant impact on minimizing or exacerbating oxidative stress.
Examine the effect of age, BMI, diet, physical activity and smoking status on the activity of erythrocyte antioxidant enzymes catalase, glutathione reductase, glutathione peroxidase glutathione S-transferase, superoxide dismutase and glutathione concentrations in healthy women.
Material and methods
This study included 98 healthy women aged between 20 and 65 years. All women underwent anthropometric tests: body weight, height, hip and waist circumference. Antioxidant activity in erythrocytes was measured by spectrophotometric methods.
Catalase activity increased significantly with age (p<0.001), while superoxide dismutase activities and glutathione decreased with age (p =0.008, p =0.023, respectively). Women with a lower BMI (emaciation) had higher superoxide dismutase activity than those in the first degree of obesity (p = 0.009
1. Increased catalase activity with age may be a sign of a large amount of hydrogen peroxide, resulting from poorly functioning antioxidant systems in older age. 2. Decreased superoxide dismutase activity with age may indicate inactivation of this enzyme by excessive hydrogen peroxide, as well as glycation of superoxide dismutase molecules or reactions with lipid peroxidation products, the intensity of which increases with age. 3. The negative correlation between superoxide dismutase activity and BMI index indicates reduced enzymatic activity in obese subjects, despite increased ROS production by adipose tissue.