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Primary research
miR-138-5p inhibits the metastasis of prostate cancer by targeting FOXC1
Xin Chen
Chifengshi Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6418-8178
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Cancer Cell International.
Background: This study aimed to uncover the regulatory effect of miR-138-5p on the metastasis of PCa cells, and further explore the potential regulatory mechanisms via regulating FOXC1. Methods: 60 pairs tumor specimens from PCa patients were collected to determine the expression level of miR-138-5p by qRT-PCR. Subsequently, over-expression of miR-138-5p were established to explore the proliferation and metastasis of miR-138-5p in PCa cell lines was analyzed by CCK-8, Tranwell assay and Wounding healing assay, respectively. Bioinformatics analysis and luciferase reporter gene assay were performed to search for the target genes of miR-138-5p, and FOXC1 was selected. Finally, the biological role of miR-138-5p and FOXC1 in the progression of PCa was clarified by a series of rescue experiments. Results: The results of qRT-PCR revealed that miR-138-5p was lowly expressed in PCa tissues and cell lines. Besdies, these PCa patients with low-miR-138-5p had a higher Gleason score, lymph node metastasis, bone metastasis and poor prognosis of PCa, compared with the patients with high-miR-138-5p. Over-expression of miR-138-5p inhibited the viability, migratory and invasive capacities of PC-3 and DU-145 cells. Bioinformatics analysis and luciferase reporter gene assay suggested that FOXC1 was predicted to be the target of miR-138-5p. Moreover, FOXC1 level was negatively correlated to that of miR-138-5p in PCa tissues. Importantly, FOXC1 could reverse miR-138-5p mimic induced-inhibition of PCa malignant progression. Conclusions: Downregulated miR-138-5p was closely associated with Gleason score, distant metastasis and poor prognosis of PCa patients. In addition, miR-138-5p alleviated the malignant progression of PCa by targeting and downregulating FOXC1.
Keywords
miR-138-5p; FOXC1; Prostate cancer; Metastasis
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CURRENT STATUS: Published
Published
On 09 Jul, 2020
No community comments so far
Editorial decision: Accept
On 24 Jun, 2020
Editor assigned
On 24 Jun, 2020
Submission checks complete
On 23 Jun, 2020
Editor invited
On 23 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 17 Jun, 2020
Reviewers invited
Invitations sent on 08 Jun, 2020
Editor assigned
On 04 Jun, 2020
Editor invited
On 03 Jun, 2020
Submission checks complete
On 03 Jun, 2020
Version 1
Posted 10 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 22 May, 2020
Review #2 received
Received 03 May, 2020
Reviewer #2 agreed
On 22 Apr, 2020
Review #1 received
Received 09 Apr, 2020
Reviewer #1 agreed
On 21 Mar, 2020
Editor assigned
On 09 Mar, 2020
Reviewers invited
Invitations sent on 09 Mar, 2020
Editor invited
On 08 Mar, 2020
Submission checks complete
On 04 Mar, 2020
First submitted
On 03 Mar, 2020
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This preprint is under consideration at Cancer Cell International. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Primary research
miR-138-5p inhibits the metastasis of prostate cancer by targeting FOXC1
Xin Chen
Chifengshi Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6418-8178
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Published
On 09 Jul, 2020
No community comments so far
Editorial decision: Accept
On 24 Jun, 2020
Editor assigned
On 24 Jun, 2020
Submission checks complete
On 23 Jun, 2020
Editor invited
On 23 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 17 Jun, 2020
Reviewers invited
Invitations sent on 08 Jun, 2020
Editor assigned
On 04 Jun, 2020
Editor invited
On 03 Jun, 2020
Submission checks complete
On 03 Jun, 2020
Version 1
Posted 10 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 22 May, 2020
Review #2 received
Received 03 May, 2020
Reviewer #2 agreed
On 22 Apr, 2020
Review #1 received
Received 09 Apr, 2020
Reviewer #1 agreed
On 21 Mar, 2020
Editor assigned
On 09 Mar, 2020
Reviewers invited
Invitations sent on 09 Mar, 2020
Editor invited
On 08 Mar, 2020
Submission checks complete
On 04 Mar, 2020
First submitted
On 03 Mar, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Cancer Cell International.
Background: This study aimed to uncover the regulatory effect of miR-138-5p on the metastasis of PCa cells, and further explore the potential regulatory mechanisms via regulating FOXC1. Methods: 60 pairs tumor specimens from PCa patients were collected to determine the expression level of miR-138-5p by qRT-PCR. Subsequently, over-expression of miR-138-5p were established to explore the proliferation and metastasis of miR-138-5p in PCa cell lines was analyzed by CCK-8, Tranwell assay and Wounding healing assay, respectively. Bioinformatics analysis and luciferase reporter gene assay were performed to search for the target genes of miR-138-5p, and FOXC1 was selected. Finally, the biological role of miR-138-5p and FOXC1 in the progression of PCa was clarified by a series of rescue experiments. Results: The results of qRT-PCR revealed that miR-138-5p was lowly expressed in PCa tissues and cell lines. Besdies, these PCa patients with low-miR-138-5p had a higher Gleason score, lymph node metastasis, bone metastasis and poor prognosis of PCa, compared with the patients with high-miR-138-5p. Over-expression of miR-138-5p inhibited the viability, migratory and invasive capacities of PC-3 and DU-145 cells. Bioinformatics analysis and luciferase reporter gene assay suggested that FOXC1 was predicted to be the target of miR-138-5p. Moreover, FOXC1 level was negatively correlated to that of miR-138-5p in PCa tissues. Importantly, FOXC1 could reverse miR-138-5p mimic induced-inhibition of PCa malignant progression. Conclusions: Downregulated miR-138-5p was closely associated with Gleason score, distant metastasis and poor prognosis of PCa patients. In addition, miR-138-5p alleviated the malignant progression of PCa by targeting and downregulating FOXC1.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5