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Research article
Guiping Zhao
Foshan University
Corresponding Author
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Background: The body distribution with high intramuscular fat and low abdominal fat is ideal goal for broiler breeding. Preadipocytes with different origins have differences in metabolism and gene expression. This transcriptome analysis of intramuscular preadipocytes (DIMPs) and adipose tissue-derived preadipocytes (DAFPs) is aim to explore the characteristics in lipid deposition of different chicken preadipocytes by dedifferentiation in vitro.
Results: Compared to DIMFPs, the lipid content was increased (P <0.05) in DAFPs after two days with 100% confluence. Moreover, 66 DEGs of lipid metabolism were screened, which are involved in the adipocyte differentiation, fatty acid transport and fatty acid synthesis, lipid stabilization, and lipolysis. Among them, the representative CEBPA, DGKH, DGKQ, DGKD, FADS1L1, SCD, SCD5, and PPARG were down-regulated, but CIDEC, ELOVL1, ELOVL6, FABP3, FABP4, FADS6, LPL, MOGAT1, PLIN3, PLIN4, RBP7, and RXRG genes were up-regulated (P < 0.05 or P < 0.01) in the DAFPs, showing the same pattern with the lipid content. Based on the known DEGs, the well-known pathways affecting lipid metabolism (MAPK-, TGF beta-, Calcium-, PPAR signaling pathway) were enriched, which may also contribute to the regulation of lipid deposition.
Conclusions: Our data suggest that the difference of lipid deposition between DIMPs and DAFPs of chicken in vitro. The lipid content was significantly increased in DAFPs by the up-regulation of genes on cellular uptake of fatty acids through medication of MAPK-, TGF beta-, Calcium-, and PPAR signaling pathways. These findings provide new insights into the regulation of tissue-specific fat deposition and optimizing body fat distribution in broilers.
Keywords
Dedifferentiated preadipocytes, Different tissue derivation, lipid deposition, DEGs, Chicken
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CURRENT STATUS: Published
View the published article at BMC Genomics.
No community comments so far
Editorial decision: Minor revision
On 02 Feb, 2021
Review #2 received
Received 21 Jan, 2021
Reviewer #2 agreed
On 15 Jan, 2021
Review #1 received
Received 10 Jan, 2021
Reviewer #1 agreed
On 09 Jan, 2021
Reviewers invited
Invitations sent on 09 Jan, 2021
Editor assigned
On 05 Jan, 2021
Submission checks complete
On 05 Jan, 2021
Editor invited
On 05 Jan, 2021
Version 1
Posted 08 Sep, 2020
No comments provided
Editorial decision: Major revision
On 26 Sep, 2020
Reviewer #5 agreed
On 19 Sep, 2020
Review #2 received
Received 18 Sep, 2020
Reviewer #3 agreed
On 18 Sep, 2020
Reviewer #4 agreed
On 18 Sep, 2020
Review #3 received
Received 18 Sep, 2020
Review #1 received
Received 18 Sep, 2020
Reviewer #2 agreed
On 15 Sep, 2020
Reviewer #1 agreed
On 15 Sep, 2020
Reviewers invited
Invitations sent on 13 Sep, 2020
Editor assigned
On 23 Jul, 2020
Submission checks complete
On 22 Jul, 2020
Editor invited
On 22 Jul, 2020
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Subject Areas
Epigenetics & Genomics
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Genomics.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Guiping Zhao
Foshan University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Genomics.
No community comments so far
Editorial decision: Minor revision
On 02 Feb, 2021
Review #2 received
Received 21 Jan, 2021
Reviewer #2 agreed
On 15 Jan, 2021
Review #1 received
Received 10 Jan, 2021
Reviewer #1 agreed
On 09 Jan, 2021
Reviewers invited
Invitations sent on 09 Jan, 2021
Editor assigned
On 05 Jan, 2021
Submission checks complete
On 05 Jan, 2021
Editor invited
On 05 Jan, 2021
Version 1
Posted 08 Sep, 2020
No comments provided
Editorial decision: Major revision
On 26 Sep, 2020
Reviewer #5 agreed
On 19 Sep, 2020
Review #2 received
Received 18 Sep, 2020
Reviewer #3 agreed
On 18 Sep, 2020
Reviewer #4 agreed
On 18 Sep, 2020
Review #3 received
Received 18 Sep, 2020
Review #1 received
Received 18 Sep, 2020
Reviewer #2 agreed
On 15 Sep, 2020
Reviewer #1 agreed
On 15 Sep, 2020
Reviewers invited
Invitations sent on 13 Sep, 2020
Editor assigned
On 23 Jul, 2020
Submission checks complete
On 22 Jul, 2020
Editor invited
On 22 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Genomics.
Background: The body distribution with high intramuscular fat and low abdominal fat is ideal goal for broiler breeding. Preadipocytes with different origins have differences in metabolism and gene expression. This transcriptome analysis of intramuscular preadipocytes (DIMPs) and adipose tissue-derived preadipocytes (DAFPs) is aim to explore the characteristics in lipid deposition of different chicken preadipocytes by dedifferentiation in vitro.
Results: Compared to DIMFPs, the lipid content was increased (P <0.05) in DAFPs after two days with 100% confluence. Moreover, 66 DEGs of lipid metabolism were screened, which are involved in the adipocyte differentiation, fatty acid transport and fatty acid synthesis, lipid stabilization, and lipolysis. Among them, the representative CEBPA, DGKH, DGKQ, DGKD, FADS1L1, SCD, SCD5, and PPARG were down-regulated, but CIDEC, ELOVL1, ELOVL6, FABP3, FABP4, FADS6, LPL, MOGAT1, PLIN3, PLIN4, RBP7, and RXRG genes were up-regulated (P < 0.05 or P < 0.01) in the DAFPs, showing the same pattern with the lipid content. Based on the known DEGs, the well-known pathways affecting lipid metabolism (MAPK-, TGF beta-, Calcium-, PPAR signaling pathway) were enriched, which may also contribute to the regulation of lipid deposition.
Conclusions: Our data suggest that the difference of lipid deposition between DIMPs and DAFPs of chicken in vitro. The lipid content was significantly increased in DAFPs by the up-regulation of genes on cellular uptake of fatty acids through medication of MAPK-, TGF beta-, Calcium-, and PPAR signaling pathways. These findings provide new insights into the regulation of tissue-specific fat deposition and optimizing body fat distribution in broilers.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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